高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 287.

• Chromatography and Separation Sciences • 上一篇    下一篇

The Effect of Secondary Chromatography on Quantitative TLC

Irena Vovk, Mirko Prosek   

  1. National Institute of Chemistry, Hcjdrihova 19, 1000 Ljubijana, Slovenia
  • 出版日期:1999-12-31 发布日期:1999-12-31

The Effect of Secondary Chromatography on Quantitative TLC

Irena Vovk, Mirko Prosek   

  1. National Institute of Chemistry, Hcjdrihova 19, 1000 Ljubijana, Slovenia
  • Online:1999-12-31 Published:1999-12-31

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摘要：

Most of TLC analysis are performed in the UV and visible spectral region by measuring reflectance with a slit-scanning densitometer or a CCD camera (1-3). However, such measurements are restricted only to the surface of the TLC plate and do not take into account the vertical sub-surface concentration distribution governed by secondary chromatography (4-7) within the sorbent. It is therefore important to investigate effects connected with secondary chromatography in order to reveal their origin, and to. estimate their impact on the results of TLC measurements and their reproducibility.

Abstract:

Most of TLC analysis are performed in the UV and visible spectral region by measuring reflectance with a slit-scanning densitometer or a CCD camera (1-3). However, such measurements are restricted only to the surface of the TLC plate and do not take into account the vertical sub-surface concentration distribution governed by secondary chromatography (4-7) within the sorbent. It is therefore important to investigate effects connected with secondary chromatography in order to reveal their origin, and to. estimate their impact on the results of TLC measurements and their reproducibility.

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