高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 267.

• Chromatography and Separation Sciences • 上一篇    下一篇

New Absorption Measurement Technique for a Super-thin Cell and its Application as a CE Detector

YANG Peng-Yuan, XU Chong-Feng   

  1. Department of Chemistry, Fudan University, Shanghai 200433, P. R. China
  • 出版日期:1999-12-31 发布日期:1999-12-31

New Absorption Measurement Technique for a Super-thin Cell and its Application as a CE Detector

YANG Peng-Yuan, XU Chong-Feng   

  1. Department of Chemistry, Fudan University, Shanghai 200433, P. R. China
  • Online:1999-12-31 Published:1999-12-31

PDF (PC)

摘要：

There are some increasing needs to consider the light absorption of a super-thin optical path. For example, in the areas of microelectronics and mechanics system (MEMS),it is possible now to fabricate an absorption flow cell in a few nm or μm scale for the optical path. In the other area of molecule separation, chemists and biochemists often apply the high-performance capillary electrophoresis (HPCE) to separate proteins and other big bio-molecules. The commonly used detector in HPCE is based on the UV-VIS light absorption, and has a thin cell in a diameter of 20-50 μm with the detection limit of 10-5 to 10-6 mol/L. It is understood that the problems associated with such a thin absorption cell are the low sensitivity and worse signal-to-noise (S/N) ratio. These problems restrict many useful applications of the super-thin cell in various purposes of light absorption measurements.

Abstract:

There are some increasing needs to consider the light absorption of a super-thin optical path. For example, in the areas of microelectronics and mechanics system (MEMS),it is possible now to fabricate an absorption flow cell in a few nm or μm scale for the optical path. In the other area of molecule separation, chemists and biochemists often apply the high-performance capillary electrophoresis (HPCE) to separate proteins and other big bio-molecules. The commonly used detector in HPCE is based on the UV-VIS light absorption, and has a thin cell in a diameter of 20-50 μm with the detection limit of 10-5 to 10-6 mol/L. It is understood that the problems associated with such a thin absorption cell are the low sensitivity and worse signal-to-noise (S/N) ratio. These problems restrict many useful applications of the super-thin cell in various purposes of light absorption measurements.

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