高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 242.

• Chromatography and Separation Sciences • 上一篇    下一篇

Determination of Heavy Polycyclic Aromatic Hydrocarbons (PAH) in Drinking Water, Ambient Air, Soils, Sediments and Food Stuffs by HPLC Method with Fluorescence Detection

Dmitry B. Gladilovich, Natalia A. Majorova, Jaroslav S. Kamentzev   

  1. Science and Research Firm"Ltimex Ltd", St. Petersburg, 198005 Russia
  • 出版日期:1999-12-31 发布日期:1999-12-31

Determination of Heavy Polycyclic Aromatic Hydrocarbons (PAH) in Drinking Water, Ambient Air, Soils, Sediments and Food Stuffs by HPLC Method with Fluorescence Detection

Dmitry B. Gladilovich, Natalia A. Majorova, Jaroslav S. Kamentzev   

  1. Science and Research Firm"Ltimex Ltd", St. Petersburg, 198005 Russia
  • Online:1999-12-31 Published:1999-12-31

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摘要：

The methods for the determination of some heavy PAH in different objects by HPLC method with fluorescence detection have been developed. The following conditions for the separation of PAH have been chosen:The column 150 mm length, internal diameter 2.1 mm filled by reversible-phase sorbent Zorbax ODS (5 μ),mobile phase acetonutrile/water (80:20), the injected volume of sample concentrate 10 μ L, the velocity of mobile phase 200 μ L.min. The excitation region is 290-300 nm, the emission one is 350-420 nm. Under condition chosen the limit of detection are 5 pg for benz(a)pyrene,10 pg for pyrene and chrysene3 pg for benz(k)fluoranthene, and 20 pg for anthracene. The dynamic range is 3 order of magnitude. The reproducibility of peak highs is better than 5%.

Abstract:

The methods for the determination of some heavy PAH in different objects by HPLC method with fluorescence detection have been developed. The following conditions for the separation of PAH have been chosen:The column 150 mm length, internal diameter 2.1 mm filled by reversible-phase sorbent Zorbax ODS (5 μ),mobile phase acetonutrile/water (80:20), the injected volume of sample concentrate 10 μ L, the velocity of mobile phase 200 μ L.min. The excitation region is 290-300 nm, the emission one is 350-420 nm. Under condition chosen the limit of detection are 5 pg for benz(a)pyrene,10 pg for pyrene and chrysene3 pg for benz(k)fluoranthene, and 20 pg for anthracene. The dynamic range is 3 order of magnitude. The reproducibility of peak highs is better than 5%.

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