高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 235.

• Chromatography and Separation Sciences • 上一篇    下一篇

A Novel Affinity Capillary Electrophoresis for the Detection of Gene Mutation Using Immobilized Oligonucleotides-Polyacrylamide Conjugate

Yoshiki Katavama1, Yoshihisa Ozaki1, Toshihiro Ihara2, Mizuo Maeda1   

  1. 1. Dept. of Materials Phys. & Chem., Graduate School of Engineering, Kyushu University, Futaoka 812-8581, Japan;
    2. Dept. of Appl. Chem. & Biochem., Facility of Engineering, Kumamoto University, Kumamoto 860-8555, Japan
  • 出版日期:1999-12-31 发布日期:1999-12-31

A Novel Affinity Capillary Electrophoresis for the Detection of Gene Mutation Using Immobilized Oligonucleotides-Polyacrylamide Conjugate

Yoshiki Katavama1, Yoshihisa Ozaki1, Toshihiro Ihara2, Mizuo Maeda1   

  1. 1. Dept. of Materials Phys. & Chem., Graduate School of Engineering, Kyushu University, Futaoka 812-8581, Japan;
    2. Dept. of Appl. Chem. & Biochem., Facility of Engineering, Kumamoto University, Kumamoto 860-8555, Japan
  • Online:1999-12-31 Published:1999-12-31

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摘要：

It has been found that small mutations of certain genes are the definitive origin of many heritable disorders and cancers with striking development of recent molecular biology. Such new findings have taken close-up of the importance of gene mutation assays based on the difference of DNA base sequences in diagnostic or medical field Capillary electrophoresis can be a good candidate for an ideal method on such gene analysis, because the methods can be performed with trace amount of samples, high resolution and shorter running time. We have established an effect of oligonucleotide, which was introduced onto capillary inner surface, on the recognition of an overall sequence of sample DNA fragments as an affinity ligand.

Abstract:

It has been found that small mutations of certain genes are the definitive origin of many heritable disorders and cancers with striking development of recent molecular biology. Such new findings have taken close-up of the importance of gene mutation assays based on the difference of DNA base sequences in diagnostic or medical field Capillary electrophoresis can be a good candidate for an ideal method on such gene analysis, because the methods can be performed with trace amount of samples, high resolution and shorter running time. We have established an effect of oligonucleotide, which was introduced onto capillary inner surface, on the recognition of an overall sequence of sample DNA fragments as an affinity ligand.

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