高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 223.

• Chromatography and Separation Sciences • 上一篇    下一篇

Organic Profiles and Pattern Recognition Analysis for Clinical Applications

Kyoung Rae Kim   

  1. College of Pharmacy, Sungkyunkkwan University, Suwon 440-746, Korea
  • 出版日期:1999-12-31 发布日期:1999-12-31

Organic Profiles and Pattern Recognition Analysis for Clinical Applications

Kyoung Rae Kim   

  1. College of Pharmacy, Sungkyunkkwan University, Suwon 440-746, Korea
  • Online:1999-12-31 Published:1999-12-31

PDF (PC)

被引次数

36

摘要：

Efficient gas chromatographic (GC) and capillary electrophoretic (CE) profiling methods were combined with simple pattern recognition methods for the correlation between urinary organic profiles (organic acids,polyamines and nucleosides) and uterine cervical cancer.For the urinary organic acid profiles, free organic acids were recovered by dual solid-phase extraction (SPE) in anion-exchange and normal-phase partition modes after methoximation of keto acids in alkalinized urine samples, followed by conversion to tert.-butyldimethylsilyl derivatives for the direct analysis by dual-capillary column GC.

Abstract:

Efficient gas chromatographic (GC) and capillary electrophoretic (CE) profiling methods were combined with simple pattern recognition methods for the correlation between urinary organic profiles (organic acids,polyamines and nucleosides) and uterine cervical cancer.For the urinary organic acid profiles, free organic acids were recovered by dual solid-phase extraction (SPE) in anion-exchange and normal-phase partition modes after methoximation of keto acids in alkalinized urine samples, followed by conversion to tert.-butyldimethylsilyl derivatives for the direct analysis by dual-capillary column GC.

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