高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 200.

• Chromatography and Separation Sciences • 上一篇    下一篇

Capillary Electrophoresis of Recombinant Human EPO

LIU Xiao-Da1, DING Xiao-Ping1, WANG Quan-Li1, Dong Fang-Ting2, Ma Li-Ren2   

  1. 1. Beijing Institute of Transfusion Medicine, Beijing 100850, P. R. China;
    2. National Center of Biomedical Analysis, Beijing 100850, P. R. China
  • 出版日期:1999-12-31 发布日期:1999-12-31

Capillary Electrophoresis of Recombinant Human EPO

LIU Xiao-Da1, DING Xiao-Ping1, WANG Quan-Li1, Dong Fang-Ting2, Ma Li-Ren2   

  1. 1. Beijing Institute of Transfusion Medicine, Beijing 100850, P. R. China;
    2. National Center of Biomedical Analysis, Beijing 100850, P. R. China
  • Online:1999-12-31 Published:1999-12-31

PDF (PC)

被引次数

56

摘要：

Capillary electrophoresis (CE) provides a new analytical tool for the separation of proteins, and almost all traditional modes of electrophoresis can be carry out in CE. But serious adsorption of proteins on capillary wall prohibited the proper separation. Three main approaches are used to overcome adsorption and control electroosmotic flow, (1) buffer of high or low pH,high salt concentration and additives, (2) pre-adsorption of neutral or charged macromolecules on the capillary wall and (3) chemically bonded coatings which are expected to give the best shield of silanol groups present on bare silica by vaious hydrophilic polymers. Capillaries coated with linear polyacrylamide represent the most successful approach available to date. Cross-linkage of polyacrylamide coating is desired to increase its stability.

Abstract:

Capillary electrophoresis (CE) provides a new analytical tool for the separation of proteins, and almost all traditional modes of electrophoresis can be carry out in CE. But serious adsorption of proteins on capillary wall prohibited the proper separation. Three main approaches are used to overcome adsorption and control electroosmotic flow, (1) buffer of high or low pH,high salt concentration and additives, (2) pre-adsorption of neutral or charged macromolecules on the capillary wall and (3) chemically bonded coatings which are expected to give the best shield of silanol groups present on bare silica by vaious hydrophilic polymers. Capillaries coated with linear polyacrylamide represent the most successful approach available to date. Cross-linkage of polyacrylamide coating is desired to increase its stability.

TrendMD: