高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 194.

• Chromatography and Separation Sciences • 上一篇    下一篇

A Method for the Determination of Temperature inside Capillaries for Electrophoresis

ZHANG Hong-Yi1,2, HU Zhi-De2, WANG Rui3   

  1. 1. Department of Chemistry, Hebei University, Baoding, 071002, P. R. China;
    2. Department of Chemistry, Lanzhou Unversity, Lanzhou, 730000, P. R. China;
    3. Department of Biology, Lanzhou University, Lanzhou, 730000, P. R. China
  • 出版日期:1999-12-31 发布日期:1999-12-31

A Method for the Determination of Temperature inside Capillaries for Electrophoresis

ZHANG Hong-Yi1,2, HU Zhi-De2, WANG Rui3   

  1. 1. Department of Chemistry, Hebei University, Baoding, 071002, P. R. China;
    2. Department of Chemistry, Lanzhou Unversity, Lanzhou, 730000, P. R. China;
    3. Department of Biology, Lanzhou University, Lanzhou, 730000, P. R. China
  • Online:1999-12-31 Published:1999-12-31

摘要:

Several researchers have addressed the problem of measuring the temperature inside the capillary. Terabe measured the outside temperature of the capillary with a micro thermocouple and found the temperature to be 70℃ at 25 kV for a 200 μm I.D. capillary. Unfortunately, the exact temperature was difficult to measure using Terabe's method because of an incomplete touch of the capillary with the micro thermocouple.

Abstract:

Several researchers have addressed the problem of measuring the temperature inside the capillary. Terabe measured the outside temperature of the capillary with a micro thermocouple and found the temperature to be 70℃ at 25 kV for a 200 μm I.D. capillary. Unfortunately, the exact temperature was difficult to measure using Terabe's method because of an incomplete touch of the capillary with the micro thermocouple.

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