高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 183.

• Chromatography and Separation Sciences • 上一篇    下一篇

Separation and Determination of Four Amino Acids by Micellar Electrokinetic Capillary Chromatography

ZHANG Yu-Xia, YE Ying-Zhi   

  1. Chemistry and Chemical Engineering College, Zhengzhou University, Henan Fundamental and Applied Science Institute, Zhengzhou, 450052, P. R. China
  • 出版日期:1999-12-31 发布日期:1999-12-31

Separation and Determination of Four Amino Acids by Micellar Electrokinetic Capillary Chromatography

ZHANG Yu-Xia, YE Ying-Zhi   

  1. Chemistry and Chemical Engineering College, Zhengzhou University, Henan Fundamental and Applied Science Institute, Zhengzhou, 450052, P. R. China
  • Online:1999-12-31 Published:1999-12-31

PDF (PC)

摘要：

The most methods used to determine amino acids developed in the past were via pre- or post-column derivatization of the analytes to produce a fluorescent reagent in order for detection of them by measuring the fluorescence. The detection of underivatized amino acids is also accomplished by indirect methods. In this work, micellar electrokinetic capillary chromatography(MECC) based on sodium dodecyl sulphate (SDS) was developed for the direct determination of the four amino acids, histidine,tyrosine, tryptophan and phenylalanine, by using UV-detector. The apparatus used was Model 3850 capillary electrophoresis system (ISCO,USA) with a 60cm 0.05mm I.D.fused-silica capillary,where was a detection window at a position 35 cm from the injection end of the capillary. And the detection performed by on-column measurement of ultraviolet absorption at 210nm. SP4600 integrator was used for the data acquisition and processing.

Abstract:

The most methods used to determine amino acids developed in the past were via pre- or post-column derivatization of the analytes to produce a fluorescent reagent in order for detection of them by measuring the fluorescence. The detection of underivatized amino acids is also accomplished by indirect methods. In this work, micellar electrokinetic capillary chromatography(MECC) based on sodium dodecyl sulphate (SDS) was developed for the direct determination of the four amino acids, histidine,tyrosine, tryptophan and phenylalanine, by using UV-detector. The apparatus used was Model 3850 capillary electrophoresis system (ISCO,USA) with a 60cm 0.05mm I.D.fused-silica capillary,where was a detection window at a position 35 cm from the injection end of the capillary. And the detection performed by on-column measurement of ultraviolet absorption at 210nm. SP4600 integrator was used for the data acquisition and processing.

TrendMD: