高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 179.

• Chromatography and Separation Sciences • 上一篇    下一篇

Determination of Catecholamine Concentration by Reverse-Phase Liquid Chromatography with Elecrtrochemical Detector in Plasma

ZHANG Yan-Wan, LONG Cun   

  1. Department of Anesthesiology and Cardicpulmonary Bypass, Fu Wai Hospital, Beijing, 100037 P. R. China
  • 出版日期:1999-12-31 发布日期:1999-12-31

Determination of Catecholamine Concentration by Reverse-Phase Liquid Chromatography with Elecrtrochemical Detector in Plasma

ZHANG Yan-Wan, LONG Cun   

  1. Department of Anesthesiology and Cardicpulmonary Bypass, Fu Wai Hospital, Beijing, 100037 P. R. China
  • Online:1999-12-31 Published:1999-12-31

摘要:

The plasma catecholamine include mainly norepinephrine(NE),epinephrine(E) and dopamine (DA). The catecholamines(CA) are nervous mediator which are synthesized and release from sympathetic system. It is significant for diagnosing disease and researching surgical stress in clinic that determine the concentration of catecholamine in plasma.The analytic method had traditionally radioactive enzyme, fluorescent spectrometry and high performance liquid chromatography(HPLC). This paper describe an analytical technique that determine the concentration of NE, E and DA in plasma using HPLC-ED. The method is sensitive, fast and accuracy,and can be applied for the clinical diagnosis and laboratory research.

Abstract:

The plasma catecholamine include mainly norepinephrine(NE),epinephrine(E) and dopamine (DA). The catecholamines(CA) are nervous mediator which are synthesized and release from sympathetic system. It is significant for diagnosing disease and researching surgical stress in clinic that determine the concentration of catecholamine in plasma.The analytic method had traditionally radioactive enzyme, fluorescent spectrometry and high performance liquid chromatography(HPLC). This paper describe an analytical technique that determine the concentration of NE, E and DA in plasma using HPLC-ED. The method is sensitive, fast and accuracy,and can be applied for the clinical diagnosis and laboratory research.

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