高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 164.

• Electroanalytical Chemistry • 上一篇    下一篇

Protein Structural Characterization by Scanning Tunneling Microscopy with Electrochemistry

WANG Er-Kang   

  1. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China
  • 出版日期:1999-12-31 发布日期:1999-12-31
  • 基金资助:

    supported by the National Natural Science Foundation of China.

Protein Structural Characterization by Scanning Tunneling Microscopy with Electrochemistry

WANG Er-Kang   

  1. Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China
  • Online:1999-12-31 Published:1999-12-31
  • Supported by:

    supported by the National Natural Science Foundation of China.

PDF (PC)

被引次数

7

摘要：

The structure characterization of native and unfolded enzymes and redox proteins like hemoglobin(Hb), myoglobin(Mb), cytochrome c and beef liver catalase etc. by STM on an electrochemically prepared HOPG surface has been studied with the help of electrodeposition. The capability of electrochemical method to prepare stable Hb samples for STM image and for unfolding of this protein has been successfully developed. STM images show an oval-shaped pattern for the native structure of Hb and two dimers consisting of one Hb molecule is clearly discerned. The dimension of folded molecule is determined as 6.4×5.3×0.7 nm3.

Abstract:

The structure characterization of native and unfolded enzymes and redox proteins like hemoglobin(Hb), myoglobin(Mb), cytochrome c and beef liver catalase etc. by STM on an electrochemically prepared HOPG surface has been studied with the help of electrodeposition. The capability of electrochemical method to prepare stable Hb samples for STM image and for unfolding of this protein has been successfully developed. STM images show an oval-shaped pattern for the native structure of Hb and two dimers consisting of one Hb molecule is clearly discerned. The dimension of folded molecule is determined as 6.4×5.3×0.7 nm3.

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