高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 157.

• Electroanalytical Chemistry • 上一篇    下一篇

Immunoassay of α-l-Fetoprotein by Enzyme-linked Differential Pulse Voltammetry

JU Huang-Xian1, CHEN Feng1, Xiao Yi1, CHEN Hong-Yuan1, YAN Gui-Feng2   

  1. 1. Department of Chemistry, Nanjing University, Nanjing 210093, P. R. China;
    2. Department of Medical Examination, Cancer Hospital and Institute of Jiangsu Province, Nanjing 210009, P. R. China
  • 出版日期:1999-12-31 发布日期:1999-12-31
  • 基金资助:

    supported by Scientific Research Foundation for Returned Overseas Chinese Scholars, The Ministry of Education of China.

Immunoassay of α-l-Fetoprotein by Enzyme-linked Differential Pulse Voltammetry

JU Huang-Xian1, CHEN Feng1, Xiao Yi1, CHEN Hong-Yuan1, YAN Gui-Feng2   

  1. 1. Department of Chemistry, Nanjing University, Nanjing 210093, P. R. China;
    2. Department of Medical Examination, Cancer Hospital and Institute of Jiangsu Province, Nanjing 210009, P. R. China
  • Online:1999-12-31 Published:1999-12-31
  • Supported by:

    supported by Scientific Research Foundation for Returned Overseas Chinese Scholars, The Ministry of Education of China.

PDF (PC)

摘要：

α-1-Fetoprotein (AFP) is an important tumor marker for the diagnosis,and even early detection of original liver carcinoma. In clinical practice its determination is only performed with IRMA or spectrophotometric ELISA. In comparison with IRMA, the later is a more welcome procedure and has been widely used to, detect AFP. However, its sensitivity is inadequate in many assays due to the influence of specimen color and some experimental factors. This work uses a gold disk electrode (0.5 mm diameter) as working electrode and improves the sensitivity detecting AFP for about ten times.

Abstract:

α-1-Fetoprotein (AFP) is an important tumor marker for the diagnosis,and even early detection of original liver carcinoma. In clinical practice its determination is only performed with IRMA or spectrophotometric ELISA. In comparison with IRMA, the later is a more welcome procedure and has been widely used to, detect AFP. However, its sensitivity is inadequate in many assays due to the influence of specimen color and some experimental factors. This work uses a gold disk electrode (0.5 mm diameter) as working electrode and improves the sensitivity detecting AFP for about ten times.

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