高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 151.

• Electroanalytical Chemistry • 上一篇    下一篇

Biosensors Based on the Oxygen Electrode Covered with Both Enzyme-Immobilized Fibroin and Acetate Cellulose Membranes

ZHANG Yu-Oing1, GU Ren-Ao2   

  1. 1. Biotech lab for Silkworm & Silk, Suzhou University, Suzhou 215151;
    2. Department of Chemistry, Suzhou University, Suzhou 215006, P. R. China
  • 出版日期:1999-12-31 发布日期:1999-12-31

Biosensors Based on the Oxygen Electrode Covered with Both Enzyme-Immobilized Fibroin and Acetate Cellulose Membranes

ZHANG Yu-Oing1, GU Ren-Ao2   

  1. 1. Biotech lab for Silkworm & Silk, Suzhou University, Suzhou 215151;
    2. Department of Chemistry, Suzhou University, Suzhou 215006, P. R. China
  • Online:1999-12-31 Published:1999-12-31

摘要:

Based on uricase-immobilized silk fibroin membranes and oxygen electrode, an amperometric biosensor for uric acid in flow-injection analysis has been developed. In the sensor, We first introduced a tailing inhibitor-ion pair reagent into a buffer system so as to eliminate all interference from hemacyte,macromolecules and small molecular weight charged species besides electroactive specie ascorbate in complex matrices.

Abstract:

Based on uricase-immobilized silk fibroin membranes and oxygen electrode, an amperometric biosensor for uric acid in flow-injection analysis has been developed. In the sensor, We first introduced a tailing inhibitor-ion pair reagent into a buffer system so as to eliminate all interference from hemacyte,macromolecules and small molecular weight charged species besides electroactive specie ascorbate in complex matrices.

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