高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (S1): 133.

• Electroanalytical Chemistry • 上一篇    下一篇

Diagnostic Applications of Electrochemical DNA Biosensor

Masamichi Nakayama1, Hiroshi Irie1, Yoshiki Katayama1, Mizuo Maeda1, Toshihiro Ihara2, Koji Nakano3   

  1. 1. Dept, of Materials Phys. & Chem., Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan;
    2. Dept, of Appl. Chem. and Biochem., Faculty of Engineering, Kumamoto University, Kumamoto 860-8555, Japan;
    3. Dept, of Chem. Systems & Engin., Graduate School of Engineering, Kyushu Univ., Fukuoka 812-8581, Japan
  • 出版日期:1999-12-31 发布日期:1999-12-31

Diagnostic Applications of Electrochemical DNA Biosensor

Masamichi Nakayama1, Hiroshi Irie1, Yoshiki Katayama1, Mizuo Maeda1, Toshihiro Ihara2, Koji Nakano3   

  1. 1. Dept, of Materials Phys. & Chem., Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan;
    2. Dept, of Appl. Chem. and Biochem., Faculty of Engineering, Kumamoto University, Kumamoto 860-8555, Japan;
    3. Dept, of Chem. Systems & Engin., Graduate School of Engineering, Kyushu Univ., Fukuoka 812-8581, Japan
  • Online:1999-12-31 Published:1999-12-31

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被引次数

5

摘要：

The replication of DNA and its transcription to RNA is one of the most remarkable examples of molecular recognition-a single-stranded DNA molecule seeks out for and hybridizes with its complementary strand. On the other hand,double-stranded DNA can interact with various molecules and ions, eg. intercalation and binding of repressor proteins. Thus, DNA can be considered as a 'receptor' in the analytical sense and should find ways in biosensor applications. From this viewpoint,we have been investigating an electrochemcial DNA biosensor based on an immobilization chemistry of DNA onto an electrode surface. In the present paper, we will report two examples of diagnostic applications of the DNA biosensor.

Abstract:

The replication of DNA and its transcription to RNA is one of the most remarkable examples of molecular recognition-a single-stranded DNA molecule seeks out for and hybridizes with its complementary strand. On the other hand,double-stranded DNA can interact with various molecules and ions, eg. intercalation and binding of repressor proteins. Thus, DNA can be considered as a 'receptor' in the analytical sense and should find ways in biosensor applications. From this viewpoint,we have been investigating an electrochemcial DNA biosensor based on an immobilization chemistry of DNA onto an electrode surface. In the present paper, we will report two examples of diagnostic applications of the DNA biosensor.

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