高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (2): 264.

• 论文 • 上一篇    下一篇

多肽IS4溶液构象的进一步NMR研究

涂光忠1, 苗振伟2, 张日清1, 罗雪春1, 蒋云2, 赵南明1   

  1. 1. 生物膜与生物膜工程国家重点实验室清华大学生物科学与技术系, 北京 100084;
    2. 北京大学化学与分子工程学院, 北京 100871
  • 收稿日期:1998-01-19 出版日期:1999-02-24 发布日期:1999-02-24
  • 通讯作者: 涂光忠,男,33岁,副研究员,现在北京微量化学研究所工作.
  • 作者简介:涂光忠,男,33岁,副研究员,现在北京微量化学研究所工作.
  • 基金资助:

    国家自然科学基金攀登项目资助(批准号:39330070)

Further NMR Investigation and Conformation Study of Polypeptide IS4

TU Guang-Zhong1, MIAO Zhen-Wei2, ZHANG Ri-Qing1, LUO Xue-Chun JIANG Yun1, ZHAO Nan-Ming2   

  1. 1. Biomembrane & Biomembrane Engineering National Laboratory, Department of Biological Science & Biotechnology, Tsinghua University, , Beijing, 100084;
    2. College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871
  • Received:1998-01-19 Online:1999-02-24 Published:1999-02-24

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被引次数

3

摘要： 利用二维核磁共振技术对多肽IS4中除Ser-1外的所有残基质子进行了指认.积分NOESY谱中的相关峰可得到距离约束.二面角的约束来自偶合常数3JNHα.由慢交换质子可得到氢键的约束.在进行距离几何程序计算时利用这些约束可得到一组构象,用能量最优化程序优化后的结果表明,多肽IS4在CF3CD2OH中的构象为α-螺旋.

关键词: 多肽IS4, 2DNMR, 多肽构象, 结构计算

Abstract: Two dimensional 1HNMR technique was used to determine the solution structure of IS4 The crosspeaks volumes in NOESY spectra were used to generate protonproton distance constraints. These data, combined with hydrogen bond constraints and angle constraints, were used to obtain a set of 10 structures by program DIANA. These structures were refined by unrestrained energy minimization using standard minimization program. The results indicated that the conformation of IS4 in CF3CD2ODis of helix.

Key words: Polypeptide IS4, 2DNMR, Polypeptide conformation, Structure calculation

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