高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (2): 243.

• 论文 • 上一篇    下一篇

漆树漆酶的催化氧化作用 (ⅹⅢ)──Fe2+离子对漆酶催化氧化反应的影响

涂楚桥1, 梁宏1, 王光辉2   

  1. 1. 广西师范大学化学化工系, 桂林 541004;
    2. 武汉大学化学学院, 武汉 430072
  • 收稿日期:1998-06-22 出版日期:1999-02-24 发布日期:1999-02-24
  • 通讯作者: 涂楚桥,男,29岁,硕士,讲师.
  • 作者简介:涂楚桥,男,29岁,硕士,讲师.
  • 基金资助:

    广西自然科学基金

Catalyzed Oxidation by Rhus Vernicifera Laccase(ⅹⅢ) Effects of Fe2+Ion on the Catalytic Oxidation by Laccase

TU Chu-Qiao1, LIANG Hong1, WANG Guang-Hui2   

  1. 1. Department of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, 541004;
    2. College of Chemistry, Wuhan University, Wuhan, 430072
  • Received:1998-06-22 Online:1999-02-24 Published:1999-02-24

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摘要： 以5,6-二溴-2,3-二氰基氢醌为底物,在pH4.50条件下,用分光光度法考察了FeSO4对漆树漆酶催化氧化反应的影响.结果表明,在本文实验条件下SO42-离子对酶促反应的影响基本可以忽略,而Fe2+离子对漆酶的催化氧化反应则有明显的竞争性抑制作用.研究证实,Fe2+离子的抑制作用是通过它还原酶促反应的产物半醌自由基阴离子来实现的.

关键词: 漆树漆酶, Fe2+离子, 竞争性抑制作用, 分光光度法

Abstract: The effects of FeSO4on the oxidation of 5,6-dibromo 2,3-dicyanohydroquinone(DDBQH2) catalyzed by Rhus vernicifera laccase were studied spectrophotometryly under condition of pH4.50. The results show that under the condition of experiments, SO42-ion has little effect on the enzymatic reaction, but Fe2+ion has obvious inhibition, the nature of inhibition is competitive type. The results demonstrate that the inhibition is realized through the reduction of product of enzymatic reaction by Fe2+ion.

Key words: Rhus vernicifera laccase, Fe2+ion, Competitive inhibition, Spectrophotometry

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