高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (2): 204.

• 论文 • 上一篇    下一篇

药物甲氧苄氨嘧啶分子模板聚合物结合作用及选择性研究

周杰, 何锡文, 赵洁, 史慧明   

  1. 南开大学化学系, 吸附功能高分子材料国家重点实验室, 天津 300071
  • 收稿日期:1998-04-24 出版日期:1999-02-24 发布日期:1999-02-24
  • 通讯作者: 何锡文
  • 作者简介:周杰,男,35岁,博士研究生,副教授,现在山东农业大学工作.
  • 基金资助:

    国家自然科学基金(批准号:29775011);南开大学吸附分离功能高分子材料国家重点实验室资助

A Study of Binding Action and Selectivity of Trimethoprim Molecular Template Polymer

ZHOU Jie, HE Xi-Wen, ZHAO Jie, SHI Hui-Ming   

  1. Department of Chemistry, The Key Lab of Functional Polymer Materials for Adsorption and Separation, Nankai University, Tianjin, 300071
  • Received:1998-04-24 Online:1999-02-24 Published:1999-02-24

摘要: 采用分子自组装印迹技术合成了对甲氧苄氨嘧啶有高选择性的模板聚合物受体.通过Scatchard分析、1HNMR和紫外光谱研究了模板聚合物的选择结合特性.结果表明,模板聚合物主要形成一类等价结合位点,通过协同氢键作用专一地结合模板底物,其离解常数为2.22×10-4mol/L.底物的选择性结合实验表明,这种聚合物对甲氧苄氨嘧啶呈现高的选择性结合能力.

关键词: 分子自组装印迹技术, 模板聚合物, 甲氧苄氨嘧啶, 底物选择性

Abstract: Amolecularly templated synthetic polymer receptor highly selective for trimethoprim was prepared by a molecular selfassembly imprinting technique. The selective binding characteristics of the template polymer were evaluated by Scatchard analysis, 1HNMR study and Ultraviolet spectrometry. The results showed that a class of same binding sites, which could specificially bind to trimethoprim by cooperative hydrogen bonding, were formed in the polymer, and its dissociation constant was estimated to be 2.22×10-4mol/L. The selective binding experiments for substrates indicated that the polymer gave much higher affinity and selectivity for trimethoprim than for sulfanilamide and sulfapyridine. It is possiple to be a good adsorption and binding materiaLIn the selective enrichment and determination of trace trimethoprim in complex biosamples.

Key words: Molacular selfassembly imprinting technique, Template polymer, Trimethoprim, Substrate selectivity

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