高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (11): 1738.

• 论文 • 上一篇    下一篇

花粉多肽对钙调素的拮抗作用研究

曲泽川1, 宋艳玲1, 阎虎生2   

  1. 1. 北京大学化学与分子工程学院, 北京 100871;
    2. 南开大学高分子化学研究所, 天津 300071
  • 收稿日期:1999-03-17 出版日期:1999-11-24 发布日期:1999-11-24
  • 通讯作者: 宋艳玲
  • 作者简介:曲泽川,男,24岁,硕士研究生.
  • 基金资助:

    国家自然科学基金(批准号:29672002)资助课题

Studies of Pollen Peptide Antagonising Effect on Calmodulin

QU Ze-Chuan1, SONG Yan-Ling1, YAN Hu-Sheng2   

  1. 1. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;
    2. Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China
  • Received:1999-03-17 Online:1999-11-24 Published:1999-11-24

PDF (PC)

被引次数

9

摘要： 研究了在Ca2+存在下荞麦花粉肽及其类似物和丹磺酰标记的钙调素(D-CaM)的相互作用,结果表明,除肽BP-1外,都能与D-CaM结合而形成复合物。利用荧光光谱法测定了这些复合物的解离常数Kd.在所研究的多肽中以BP-13拮抗CaM作用最强,其Kd值为4.6×10-2μmol/L,抑制钙依赖性磷酸二酯酶活性的IC50为2.2μmol/L.我们还发现,当D-丙氨酸残基取代没有亲和性的BP-1中甘氨酸残基时,其亲和性明显提高。

关键词: 花粉多肽, 钙调素, 钙调素拮抗剂

Abstract: The interactions of the buckwheat pollen peptide and its analogues with dansyl labeled calmodulin(D-CaM) were studied in the presence of Ca2+. All peptides except BP-1 can associate with D-CaM to form complexes peptide-calmodulin. The dissociation constants Kdfor the complexes were determined using the fluorescence spectra. Among the tested peptides, BP-13 showed a stronger inhibitory effect on CaM, with IC50of 2.2 μmol/Land Kdof 4.6×10-2μmol/L. The effects of the hydrophobicity and the behavior being predicated to have a higher α-helical propensities on their affinities were further studied. We have found that D-alanine substitution in BP-1 results in affinity enhancement. The result will be helpful to improving our ability to design peptides possessing anticalmodulin activity.

Key words: Pollen peptide, Calmodulin, Calmodulin antagonist

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