高等学校化学学报

高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (11): 1661.

• 论文 •    下一篇

钌多吡啶配合物的合成及插入配体的位阻效应对键合DNA的影响

甄启雄1, 叶保辉1, 刘劲刚1, 计亮年1, 王雷2   

  1. 1. 中山大学化学系, 广州 510275;
    2. 广州师范大学化学系, 广州 510400
  • 收稿日期:1999-03-22 出版日期:1999-11-24 发布日期:1999-11-24
  • 通讯作者: 计亮年
  • 作者简介:甄启雄,男,34岁,博士研究生.
  • 基金资助:

    国家自然科学基金(批准号:29731030);广东省自然科学基金;中国科学院上海有机化学研究所生命有机化学国家重点实验室资助

Synthesis of Ruthenium Polypyridyl Complexes and the Steric Influence of the Intercalated Ligand on DNA-Binding

ZHEN Qi-Xiong1, YE Bao-Hui1, LIU Jin-Gang1, JI Liang-Nian1, WANG Lei2   

  1. 1. Department of Chemistry, Zhongshan University, Guangzhou 510275, China;
    2. Department of Chemistry, Normal University of Guangzhou, Guangzhou 510400, China
  • Received:1999-03-22 Online:1999-11-24 Published:1999-11-24

PDF (PC)

被引次数

52

摘要： 合成了2,3-二甲基-1,4,8,9-四氮三联苯(dmtatp)和2,3-二苯基-1,4,8,9-四氮三联苯(dptatp)两种新配体及它们与2,2′-联吡啶和钌(Ⅱ)的混配物[Ru(bpy)2dmtatp]2+(1)和[Ru(bpy)2dptatp]2+(2),用电子吸收光谱、稳态荧光、粘度测定和圆二色谱研究了配合物与小牛胸腺DNA的相互作用。结合我们以前对配合物[Ru(bpy)2tatp]2+(3)(tatp为1,4,8,9-四氮三联苯)与小牛胸腺DNA的作用研究,得出配合物与DNA的键合强度顺序为:[Ru(bpy)2tatp]2+>[Ru(bpy)2dptatp]2+>[Ru(bpy)2dmtatp]2+,这与插入配体的位阻效应的减少趋势相一致。同时,还测定了配合物与DNA的键合常数。

关键词: 多吡啶钌(Ⅱ), 小牛胸腺DNA, 插入作用, 位阻效应

Abstract: Two structurally related ligands dmtatp(2,3-dimethyl-1,4,8,9-tetraazatriphenylene), dptatap(2,3-diphenyl-1,4,8,9-tetraazatriphenylene) and their ruthenium(Ⅱ) complexes [Ru(bpy)2dmtatp]2+(1) and [Ru(bpy)2dptatp]2+(2) were synthesized and characterized. Combined with the previous study data of [Ru(bpy)2tatp]2+(3, tatp=1,4,8,9-tetraazatriphenylene) the steric influence of the intercalated ligand of these complexes on DNA-binding were investigated by electronic absorption, steady state luminescence, CDspectra and viscosity measurement. All the data suggested that the complexes bind with DNA by intercalation and the order of binding affinities is 3>2>1, consistent with the reduced steric effect of the intercalated ligand.

Key words: Ruthenium(Ⅱ) polypyridyl, Calf thymus DNA, Intercalation, Steric effect

中图分类号: 

TrendMD: