高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (8): 1219.

• 论文 • 上一篇    下一篇

微乳液组成对毛细管微乳液电动色谱的影响

吕建德, 傅小芸, 徐秀珠   

  1. 浙江大学应用化学研究所, 杭州, 310027
  • 收稿日期:1997-11-25 出版日期:1998-08-24 发布日期:1998-08-24
  • 通讯作者: 吕建德,女,56岁,高级工程师.
  • 作者简介:吕建德,女,56岁,高级工程师.
  • 基金资助:

    国家自然科学基金(批准号:29675020)资助课题.

Effects of Microemulsion Composition on Microemulsion Electrokinetic Capillary Chromatography

Lü Jian-De, FU Xiao-Yun, XU Xiu-Zhu   

  1. Institute of Applied Chemistry, Zhejiang University, Hangzhou, 310027
  • Received:1997-11-25 Online:1998-08-24 Published:1998-08-24

摘要: 研究了一组电中性芳香族化合物的毛细管微乳液电动色谱分离,考察分离介质微乳液的组成对电动色谱保留时间的影响.实验结果表明,各芳香化合物的保留时间均随表面活性剂浓度增加而增长;内相和助表面活性剂对有、无亲水基团芳香化合物的保留时间有不同的影响规律.选用微乳液组成为80mmol/L正庚烷-120mmol/LSDS-900mmol/L正丁醇-10mmol/L硼砂,样品在14min内达到完全分离,理论板数为3×105~9×105/m.

关键词: 毛细管电动色谱, 微乳液, 芳香族化合物

Abstract: A set of wide polar aromatic analytes was separated by microemulsion electrokinetic capillary chromatography(MEEKC). The effects of microemulsion compositions, including the core phases(n-hexane, n-heptane, n-octane) alkyl chain length, the concentrations of core phase heptane, surfactant sodium dodecyl sulfate and co-surfactant n-butanol, on electrokinetic separation were studied. The results showed that the surfactant had the similar effects on the retention time of all analytes. And both core phase and co-surfactant had different effects on analytes with hydrophilic functional groups and without any hydrophilic functional group. The separation mechanism of microemulsion electrokinetic chromatography was discussed. Using microemulsion consisting of 80 mmol/L n-heptane-120 mmol/L SDS-900 mmol/L n-butanol-10 mmol/Lborate, the complete separation was obtained within 14minutes. The numbers of theoretical plate ranged 3×105-9×105per meter.

Key words: Electrokinetic capillary chromatography, Microemulsion, Aromatic compound

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