高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (11): 1758.

• 论文 • 上一篇    下一篇

血管紧张素Ⅱ(AngⅡ)的溶液构象研究

王锐1, 胡晓愚1, 马亚平1, 李笃2   

  1. 1. 兰州大学生物系, 应用有机化学国家重点实验室, 兰州, 730000;
    2. BrighamYoung大学化学系, Provo, Utah, 美国
  • 收稿日期:1997-11-12 出版日期:1998-11-24 发布日期:1998-11-24
  • 通讯作者: 胡晓愚
  • 作者简介:王锐,男,35岁,博士,教授,博士生导师.
  • 基金资助:

    国家自然科学基金(批准号:29502007)、国家教育委员会博士点基金和霍英东教育基金资助课题.

Studies on the Solution Conformation of Angiotensin Ⅱ

WANG Rui1, HU Xiao-Yu1, MA Ya-Ping1, Li Du2   

  1. 1. Biochemistry Laboratory of Biology Department, State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000;
    2. Department of Chemistry, Brigham Young University, Provo, Utah, United States
  • Received:1997-11-12 Online:1998-11-24 Published:1998-11-24

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被引次数

5

摘要： 用固相多肽合成方法合成AngⅡ,并用2DNMR技术测定其在D2O与DMSO-d6中的构象.通过偶合常数、ROE效应、化学位移温度系数,确定其二面角约束、距离约束及氢键约束·结果表明,AngI在D2O与DMSO-d6中构象极为相近,呈无规卷曲状态,排除了其构象为α螺旋、310螺旋、β转角、γ转角、平行与反平行β折叠的可能性,且其在水中的构象对温度不敏感.

关键词: AngⅡ, 固相多肽合成, 2D NMR, 肽溶液构象

Abstract: Human angiotensin (AngⅡ) was obtained by solid phase method. Its solution conformations in D2Oand DMSOd6 were examined by 2D NMRtechnique. The dihedral angle constraint, distance constraint, hydrogen bond constraint were made based on coupling constants, ROE effect, temperature coefficient of chemical shifts. It is concluded that Ang Ⅱ exists in D2Oand DMSOd6 as closed similar conformations that is random coil with negligible content of α helix, 310 helix, β turns, γ turns, parallel and antiparallel β sheet. The conformation in D2Owas not sensitive to temperature.

Key words: AngⅡ, Solid phase peptide synthesis, 2D NMR, Solution conformation of peptide

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