大孔硼酸基聚苯乙烯树脂分离果葡糖研究

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (10): 1689.

大孔硼酸基聚苯乙烯树脂分离果葡糖研究

葛玉斌, 孙文田, 王树岩, 周慧, 李惟

吉林大学分子生物学系, 长春, 130023

收稿日期:1997-07-22 出版日期:1998-10-24 发布日期:1998-10-24
通讯作者: 葛玉斌.男,28岁,博士,讲师.
作者简介:葛玉斌.男,28岁,博士,讲师.
基金资助:
吉林省科学技术委员会自然科学基金资助课题.

Separation of Fructose from Fructose-Glucose Syrup Using Macroporous Dihydroxyborylated Polystyrene Resin

GE Yu-Bin, SUN Wen-Tian, WANG Shu-Yan, ZHOU Hui, LI Wei

Department of Molecular Biology, Jilin University, Changchun, 130023

Received:1997-07-22 Online:1998-10-24 Published:1998-10-24

摘要/Abstract

摘要： 通过偶氮反应将氨基苯硼酸共价连接到聚苯乙烯载体上,制备了硼酸基聚苯乙烯载体.研究了实验条件对载体吸附果糖能力及载体分离柱分离效果的影响,确定了最佳洗脱条件.以长径比为175:1的分离柱,于室温下用pH6.1的二次水以0.16mL/min的流速进行洗脱,成功地将42%的果葡糖分离,果糖纯度达到99%.

关键词: 大孔硼酸基聚苯乙烯载体, 果葡糖浆, 果葡糖分离

Abstract: Macroporous dihydroxyborylated polystyrene resin was prepared in this paper through coupling m-aminophenylboronic acid and macroporous aminopolystyrene by diazotization. Some factors which influence the absorbing ability for fructose of the resin were investigated. Some factors which affect the elution curve were also studied. Fructose was successfully separated from the mixture of glucose and fructose when using the resin as the separating medium. The purity of the obtained fructose syrup was 99%, with a concentration of 106 mg/mL. This resin may potentially be used in industry for separating glucose and fructose.

Key words: Macroporous dinitrohydroxyborylated polystyrene resin, Fructose glucose syrup, Separation of fructose and glucose (Ed.: Q,L)

中图分类号:

O631

TrendMD:

葛玉斌, 孙文田, 王树岩, 周慧, 李惟. 大孔硼酸基聚苯乙烯树脂分离果葡糖研究. 高等学校化学学报, 1998, 19(10): 1689.

GE Yu-Bin, SUN Wen-Tian, WANG Shu-Yan, ZHOU Hui, LI Wei . Separation of Fructose from Fructose-Glucose Syrup Using Macroporous Dihydroxyborylated Polystyrene Resin. Chem. J. Chinese Universities, 1998, 19(10): 1689.