高等学校化学学报

高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (8): 1303.

• 研究快报 • 上一篇    下一篇

氯化血红素作为模拟酶荧光免疫分析乙肝表面抗原

朱庆枝1, 郑雪英1, 李芳1, 许金钩1, 刘风华2, 李文友1   

  1. 1. 国家教育委员会材料和生命过程分析科学开放实验室, 厦门 361005;
    2. 厦门大学化学系,
  • 收稿日期:1997-01-30 出版日期:1997-08-24 发布日期:1997-08-24
  • 通讯作者: 许金钩.
  • 作者简介:朱庆枝, 男, 28岁, 博士研究生.
  • 基金资助:

    国家教育委员会材料和生命过程分析科学开放实验室资助

Hemin as a Peroxidase Substitute in Mimetic Enzyme Immunoassay for Hepatitis B Surface Antigen

ZHU Qing-Zhi1, ZHENG Xue-Ying1, LI Fang1, XU Jin-Gou1, LIU Feng-Hua2, LI Wen-You1   

  1. 1. The Research Laboratory of SEDC Of Analytical Science for Material and Life Chemistry, Department of Biology, Xiamen University, Xiamen, 361005;
    2. Department of Chemistry, Xiamen University, Xiamen, 361005
  • Received:1997-01-30 Online:1997-08-24 Published:1997-08-24

PDF (PC)

被引次数

9

关键词: 模拟酶荧光免疫分析, 氯化血红素, 乙肝表面抗原, 盐酸硫胺素

Abstract: Anovel mimetic enzyme immunoassay method was developed for the determina-tion of hepatitis Bsurface antigen (HBsAg) in solution. Hemin, a horseradish peroxidase substitute, was used as a labelling reagent to catalyze the reaction of thiamine, a new fluoro-genic substrate, with hydrogen peroxide at pH 8.5. In the sandwich immunoassay,anti-HBsAg antibody was coated on a 96-well plate(polystyrene), which first reacted with stan-dard HBsAg solution, and then further reacted with the fixed amount of hemin-labelled anti-HBsAg. After the two-step immunoreaction, the immunochemically adsorbed hemin-anti-HBsAg conjugate moiety was determined by measuring the fluorescence produced in a solu-tion containing thiamine and hydrogen peroxide. The flourescence intensity was directly pro-portional to the concentration of HBsAg. The calibration graph for HBsAg was linear over the range 2.5-500ng/well with a detection limit of 2.5ng/well.

Key words: Mimetic enzyme flourescence immunoassay analysis, Hemin, HBsAg, Thiamine

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