高等学校化学学报

高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (7): 1041.

• 论文 • 上一篇    下一篇

基于可见吸收信号的乳酸脱氢酶光纤传感器

王柯敏1, 林朝晖1, 蔡巍1, 陈永康2, 李慧明2   

  1. 1. 湖南大学化学化工学院, 长沙 410082;
    2. 香港浸会大学化学系, 香港
  • 收稿日期:1997-04-04 出版日期:1997-07-24 发布日期:1997-07-24
  • 通讯作者: 王柯敏, 男, 39岁, 博士, 教授, 博士导师.
  • 作者简介:王柯敏, 男, 39岁, 博士, 教授, 博士导师.
  • 基金资助:

    国家自然科学基金

A Fiber-optic Biosensor Based on Visible Absorption for the Dtermination of Lactate Dehydrogenase Activity

WANG Ke-Min1, LIN Zhao-Hui1, CAI Wei1, Chan W. H.2, Lee Albert W. M.2   

  1. 1. College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082;
    2. Department of Chemistry, Hong Kong Baptist University, Hong Kong
  • Received:1997-04-04 Online:1997-07-24 Published:1997-07-24

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被引次数

5

摘要： 报道了一种测定乳酸脱氢酶活力的基于可见吸收信号的光纤生物传感器. 在该传感体系中,通过辅酶I的氧化还原对(NAD+/NADH)将乳酸脱氢酶和心肌黄酶催化的两个脱氧过程予以耦合. 第一个脱氢过程对分析对象进行化学识别,第二个脱氢过程引起可见吸收信号的变化. 该传感器对0～400U/L的乳酸脱氢酶有线性响应关系,检测下限为48U/L. 该传感器已用于人体血清中乳酸脱氢酶活力的测定.

关键词: 光纤生物传感器, 脱氢酶活力, 乳酸脱氢酶, 心肌黄酶, 辅酶I

Abstract: Lactate dehydrogenase activities can be determined by using a fiber-optic biosen-sor based on visible absorption.In this method two dehydrogenate procedures were catalyzedby lactate dehydrogenase and diaphorase respectively, and coenzyme I (NAD+/NADH) wasintroduced into the two enzyme reactions as an electron transfer.The first dehydrogenateprocedure is for the recognition of lactate dehydrogense and the second one will show achange of absorbance- The sensing element of the biosonsor consists of draphorase covalencebound on the tip of the fiber bundle.Alinear response of the biosensor was obtained in therange of 0-400 U/Lof lactate dehydrogenase with a detection limit of 48 U/L- This biosen-sor has been used to determine lactate dehydrogenase activities in human serum.

Key words: Fiber-optic biosensor, Dehydrogenase activity, Lactate dehydrogenase, Di-aphorase, Coenzyme I

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