高等学校化学学报

高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (6): 840.

• 论文 • 上一篇    下一篇

铂配合物cis-[Pt(NH3)2(4-mepy)Cl]NO3与甲基-谷胱甘肽作用的NMR研究

陈宝卫1, StellaS. G. E. VanBoom2, JanReedljk2   

  1. 1. 北京医科大学无机教研室, 北京, 100083;
    2. 荷兰来顿大学化学系, 荷兰
  • 收稿日期:1996-06-20 出版日期:1997-06-24 发布日期:1997-06-24
  • 通讯作者: 陈宝卫, 男, 40岁, 副教授.
  • 作者简介:陈宝卫, 男, 40岁, 副教授.
  • 基金资助:

    国家教育委员会留学口国人员基金

1H NMR Study of Interaction of cis-[Pt (NH3)2 (4-methylpyridine) Cl]NO3 with S-methylglutathione

CHEN Bao-Wei1, StellaS. G. E. VanBoom2, JanReedljk2   

  1. 1. Department of Inorganic Chemistry, Beijing Medical University, Beijing, 100083;
    2. Leiden Institute of Chemistry, Leiden University, R. A. Leiden, the Netherlands
  • Received:1996-06-20 Online:1997-06-24 Published:1997-06-24

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摘要： 使用质子核磁共振方法测定了新的抗癌活性配合物cis－[Pt(NH3)2(4－mepy)Cl]+与甲基-谷胱甘肽(GS－Me)的反应及其动力学.结果表明Pt与GS－Me间形成1:1加合物.为了解结合部位,对Pt－GS－Me配合物进行pH滴定,由GS－Me的质子核磁共振谱对pH的依赖关系判断Gs－Me只通过S原子与Pt配位,而氨基和羧基不参与配位反应.由配体吡啶环质子核磁共振随时间的变化,提出了两步反应机制:首先GS－Me通过S原子与Pt配位,然后由于S配位的高度反位效应使反应的NH3活动化.通过1HNMR方法测定了第一步反应的速率常数k＝0.017L·mol－1·s-1(pH 3.1)和k＝0.013L·mol－1·s-1(pH 7.2).

关键词: 铂配合物, 甲基-谷胱甘肽, NMR

Abstract: The interaction of antitumor activity complex, cis-[Pt (NH3)2, (4-methylpyridine )Cl]No3 with S-methylglutathione (GS-Me) was determined using the method of proton NMR. The proton resonances of Pt complex and GS-Me were used as an indication of whether the reaction was completed or not. The results indicate that a Pt- to GS-Me ratio of 1:1 is required to bind all available GS-Me- The complex of Pt with GS-Me were titrated over pHrange 1-12. The pHdependence of chemical shifts of g1, g3, g4 and g5 is depicted.It is concluded that GS-Me bind to Pt via Satom and no amino and carboxyl groups coordi-nate to Pt. The reaction mechanism was discussed and the time course of reaction was recorded. The reaction of Pt with GS-Me suggests a process in two steps: the first step in-volves the formation of Pt-GS-Me adduct via Satom, then ammonia coordinated trans to Swill be lbaialized due to the high trans effect of S. The rate constant of reaction between Pt complex and GS-Me was determined by proton NMR, k=0.017 L. mol-1. s-1 (pH3.1)and 0.013 L. mol-1. s-1(pH 7.2).

Key words: Platinum complex, S-methylglutathione, 1H NMR

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