高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (5): 734.
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苗振伟, 蒋云, 李向群, 徐筱杰, 唐有祺
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MIAO Zhen-Wei, JIANG Yun, LI Xiang-Qun, XU Xiao-Jie, TANG You-Qi
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摘要: 采用本甲酸甲酯作为α-羧基的暂时性保护基,以片段缩合的方式合成了疏水性多肽──鼠脑钢通道Ⅰ的IS3片段,利用高效液相色谱对其进行了纯化,并通过了氨基酸组成分析和快原子轰击质谱鉴定.
关键词: 苯甲酸甲酯, 多肽合成, IS3片段, 片段缩合
Abstract: The IS3 segment, a peptide with 22 amino acid residues in length, of the rat brain sodium ion channel was synthesized by the fragment condensation using the phenacylester as the temporary protection group of a-carboxyl group.After removal of the protection group,the crude product was purified by HPLCfirst on Protein Parck column and then on C18 column, using 40% acetonitrile in 0.1 mol/L NH4HCO3 aqueous solution as the mobile phase.The purified IS3 segment was confirmed by amino acid analysis and FAB-MS.
Key words: Phenacylester, Peptide synthesis, IS3 segment, Fragment condensation
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苗振伟, 蒋云, 李向群, 徐筱杰, 唐有祺. 鼠脑钠通道Ⅰ的IS3片段的溶液法合成. 高等学校化学学报, 1997, 18(5): 734.
MIAO Zhen-Wei, JIANG Yun, LI Xiang-Qun, XU Xiao-Jie, TANG You-Qi. Synthesis of the IS3 Segment of the Rat Brain Sodium Ion Channel. Chem. J. Chinese Universities, 1997, 18(5): 734.
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http://www.cjcu.jlu.edu.cn/CN/Y1997/V18/I5/734