高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (5): 734.

• 论文 • 上一篇    下一篇

鼠脑钠通道Ⅰ的IS3片段的溶液法合成

苗振伟, 蒋云, 李向群, 徐筱杰, 唐有祺   

  1. 北京大学化学与分子工程学院, 北京, 100871
  • 收稿日期:1996-05-28 出版日期:1997-05-24 发布日期:1997-05-24
  • 通讯作者: 苗振伟, 男, 34岁, 博士, 副教授.
  • 作者简介:苗振伟, 男, 34岁, 博士, 副教授.
  • 基金资助:

    国家攀登计划资助

Synthesis of the IS3 Segment of the Rat Brain Sodium Ion Channel

MIAO Zhen-Wei, JIANG Yun, LI Xiang-Qun, XU Xiao-Jie, TANG You-Qi   

  1. College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871
  • Received:1996-05-28 Online:1997-05-24 Published:1997-05-24

摘要: 采用本甲酸甲酯作为α-羧基的暂时性保护基,以片段缩合的方式合成了疏水性多肽──鼠脑钢通道Ⅰ的IS3片段,利用高效液相色谱对其进行了纯化,并通过了氨基酸组成分析和快原子轰击质谱鉴定.

关键词: 苯甲酸甲酯, 多肽合成, IS3片段, 片段缩合

Abstract: The IS3 segment, a peptide with 22 amino acid residues in length, of the rat brain sodium ion channel was synthesized by the fragment condensation using the phenacylester as the temporary protection group of a-carboxyl group.After removal of the protection group,the crude product was purified by HPLCfirst on Protein Parck column and then on C18 column, using 40% acetonitrile in 0.1 mol/L NH4HCO3 aqueous solution as the mobile phase.The purified IS3 segment was confirmed by amino acid analysis and FAB-MS.

Key words: Phenacylester, Peptide synthesis, IS3 segment, Fragment condensation

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