高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (2): 207.

• 论文 • 上一篇    下一篇

预聚合法制备聚丙烯酰胺毛细管凝胶柱

胡涌刚, 邓延倬   

  1. 武汉大学分析测试中心, 武汉, 430072
  • 收稿日期:1996-04-19 出版日期:1997-02-24 发布日期:1997-02-24
  • 通讯作者: 邓延悼.
  • 作者简介:胡涌刚, 男, 26岁, 博士研究生.
  • 基金资助:

    国家自然科学基金

A New Technique of Pre-polymerization for Preparation of Polyacry-amide Gel Filled Capillaries for Capillary Gel Electrophoresis

HU Yong-Gang, DENG Yan-Zhuo   

  1. Center of Analysis and Testing, Wuhan University, Wuhan, 430072
  • Received:1996-04-19 Online:1997-02-24 Published:1997-02-24

摘要: 提出了一种温和条件下制备交联聚丙烯酸胺毛细管凝胶电泳柱的新方法──预聚合法.讨论了制备过程中的问题,获得了凝胶组成、毛细管内径及长度等不同的交联聚丙烯酸胺毛细管凝胶电泳柱.采用激光回射干涉检测技术,实现了蛋白质标准样品的毛细管凝胶电泳在柱分离检测.

关键词: 交联聚丙烯酰胺, 毛细管凝胶电泳, 预聚合

Abstract: Anovel method "pre-polymerization" for production of cross-linked polyacrylamide gel filled-capillaries was studied in detail.Many factors of this method which contribute to the ratio of success for producing the gel-filled capillaries were discussed.i.e.,characters and pureness of Acr and Bis, use level of TeMEDand AP, effect of pHvalue and other characters of buffer, time of polymerization, diameter and length of capillaries, and conditions of preservation and so on.This method was demonstrated to be applicable to the production of gel-filled capillaries with wide varieties of gel composition, capillary diameters and capillary lengh.Over 9O% ratio of success for producing the gel-filled capillaries was obtained with this method.Retroreflective laser beams interference phase-shift detection was used for on-column detection of proteins in cross-linked polyacrylamide gel capillary electrophoresis (CPAGCE) firstly.For ovalbumin, 4.2 ×10-7 mol/L(S/N=2) of detection limit was measured and 1.66 ×105 theoreticalplates was observed on the home-made CEsystem.It should prove attractive for a number of applications in separation of biological macromolecule samples with the gel-filled capillaries produced by the method of pre-polymerizatlon.

Key words: Cross-linked polyacrylamide, CGE, Prepolymerization

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