高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (12): 1966.

• 研究简报 • 上一篇    下一篇

基体辅助激光解吸电离飞行时间质谱法测定钙调素的分子量

刘德龙1, 朱智华2, 赵善楷2, 孙大业3, 杨燕生1   

  1. 1. 中山大学化学系, 广州 510275;
    2. 中山大学测试中心, 广州;
    3. 河北师范大学生物系, 石家庄 050016
  • 收稿日期:1996-11-28 出版日期:1997-12-24 发布日期:1997-12-24
  • 通讯作者: 杨燕生
  • 作者简介:刘德龙, 男, 31岁, 博士研究生.
  • 基金资助:

    国家自然科学基金

Matrix-assisted Laser Desorption Ionization Mass Spectrometry for Determination of Molecular Weight of CaMs

LlU De-Long1, ZHU Zhi-Hua2, ZHAO Shan-Kai2, SUN Da-Ye3, YANG Yan-Sheng1   

  1. 1. Department of Chemistry, Analytical and Testing Centert, Zhongshan University, Guangzhou 510275;
    2. Analytical and Testing Centert, Zhongshan University, Guangzhou 510275;
    3. Department of Biology, Hebei Normal University, Shijiazhuang 050016
  • Received:1996-11-28 Online:1997-12-24 Published:1997-12-24

关键词: 激光质谱, 钙调素, 胞外钙调素, 分子量测定

Abstract: The molecular weight of bovine brain CaM, intracellular CaMand extracellularCaMof cauliflower have been determined for the first time by Matrix-Assisted Larer Desorp tion Ionization Time of Flight Mass Spectrometry(MALDI-TOF-MS).The molecular weightof bovine brain CaM, 16699.9 Da determined by MALDI-TOF-MS, is closely ideatical with16700 Da, calculated from its primary sequence, showing the high accuracy of MALDITOF-MSand the conf1rmation of previously determined sequence.Furthermore, the molecular weight of intracellular CaMand extracellular CaMdetermined by MAlDI-TOF-MS, 17025 Da and 15582 Da, are in agreement with 17500 Da and 15000 Da determined by SDSPAGE-This agreement shows the high accuracy of MALDI-TOF-MS.In addition, themolecular weight of CaMs with this technique can be determined rapidly(less than 10 min)and sensitively.

Key words: MALDI-TOF-MS, Calmodulin, Extracellular calmodulin, Determination of molecular weight

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