高等学校化学学报

高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (11): 1797.

• 研究简报 • 上一篇    下一篇

毛细管区带电泳定量分析SD大鼠前列腺素

何金兰1, 李惠萍2, 李小戈1   

  1. 1. 湛江师范学院化学系, 湛江 524048;
    2. 广东医学院附属医院呼吸病研究室, 湛江 524001
  • 收稿日期:1996-08-01 出版日期:1997-11-24 发布日期:1997-11-24
  • 通讯作者: 何金兰, 女, 49岁, 副教授
  • 作者简介:何金兰, 女, 49岁, 副教授
  • 基金资助:

    广东省高等教育厅自然科学基金

Quantitative Analysis of Prostaglandins in SD Rats by Capillary Zone Electrophoresis

HE Jin-Lan1, LI Hui-Ping2, Ll Xiao-Ge1   

  1. 1. Department of Chemistly, Zhanjiang Normal College,Zhanjiang 524048;
    2. Respiratory Diseases ResearchLaboratory in Affiliated Hospital of Guangdong Medical College, Zhanjiang, 524001
  • Received:1996-08-01 Online:1997-11-24 Published:1997-11-24

PDF (PC)

被引次数

4

关键词: 毛细管区带电泳, 间接紫外吸收检测, 前列腺素

Abstract: This paper reports an investigation of optimizing separation and detection foranimal prostaglanadins, i.e.prostaglandins E2 (PGE2) and thromboxane B2(TXB2), bycapillary zone electrophoresis with indirect UVdetection at 254 nm.It was found that the addinon of 3 mmol/Lphosphomolybdic acid in 5 mmol/Lchromate chromate can lower the detectionlimits.The linear ranges for TXB2 and PGE2 were the same, i.e.5-80 μg/mL, theanalytical precisions (n=8) were 1.2%-1.4% and 3.5% for the measurement of the migrationtimes and for the determination with peak height, respectively.This approach wasdemonstrated in the lung of SDmouse and the results obtained agreed with those from references.

Key words: Capillary zone electrophoresis, Indirect UV detection, Prostaglendins

中图分类号: 

TrendMD: