高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (8): 1191.

• 论文 • 上一篇    下一篇

阿达玛变换显微图象分析(Ⅱ)──荧光衰减对图象和定量分析数据的影响

梅二文1, 顾文芳1, 陈观铨1, 曾云鹗2   

  1. 1. 武汉大学分析测试中心, 武汉, 430072;
    2. 武汉大学化学系, 武汉, 430072
  • 收稿日期:1994-08-23 修回日期:1995-02-16 出版日期:1995-08-24 发布日期:1995-08-24
  • 通讯作者: 梅二文,男,29岁,博士,讲师.
  • 作者简介:梅二文,男,29岁,博士,讲师.
  • 基金资助:

    国家自然科学基金

Hadamard Tsansform Microscope Image Analysis(Ⅱ)─Study on the Effect of Fluorescence Bleach on Image and Quantitative Data

MEI Er-Wen1, GU Wen-Fang1, CHEN Guan-Quan1, ZENG Yun-E2   

  1. 1. Center of Instrumental Analysis, Wuhan, 430072;
    2. Department of Chemistry, Wuhan University, Wuhan, 430072
  • Received:1994-08-23 Revised:1995-02-16 Online:1995-08-24 Published:1995-08-24

摘要: 采用计算机模拟的方法,系统地考察了试样的荧光衰减对阿达玛变换图象和定量分析数据的影响,并把阿达玛变换显微图象分析仪用于细胞内DNA的定量分析,得到了满意的结果。

关键词: 阿达玛变换, 荧光图象, 荧光衰减, DNA定量分析

Abstract: This paper reports the effect of fluorescence bleach of analytical sample on Hadamard transform image and quantitative data,which was investigated by using a computer simulation method.The instrument of Hadamard transform microscope image analysis was applied to quantitatively analyze the DNA content in plant another dust cell.the result is found satisfactory.

Key words: Hadamard transform, Fluorescence image, Fluorescence bleach, DNA quantitative analysis

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