高等学校化学学报

高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (5): 715.

• 论文 • 上一篇    下一篇

反胶团中漆树酶催化氧化性能与反应产物鉴定

杜予民, 张金梅   

  1. 武汉大学环境科学系, 武汉, 430072
  • 收稿日期:1994-05-13 修回日期:1995-02-17 出版日期:1995-05-24 发布日期:1995-05-24
  • 通讯作者: 杜予民,男,57岁,教授
  • 作者简介:杜予民,男,57岁,教授.
  • 基金资助:

    国家教育委员会自选课题

Studies on the Oxidation Performance and Reaction Product of Rhus Laccase in Reverse Micelles

DU Yu-Min, ZHANG Jin-Mei   

  1. Department of Environmental Science, Wuhan University, Wuhan, 430072
  • Received:1994-05-13 Revised:1995-02-17 Online:1995-05-24 Published:1995-05-24

PDF (PC)

被引次数

11

摘要： 研究了漆树酶在AOT/正辛烷/水反胶团中邻氨基苯酚的催化氧化性能。发现在40℃、pH=7.6、R=[H2O]/[AOT]≈18的条件下,漆树酶的活力最高,在含水量较低的反胶团中较稳定。当R=6.2,于30℃保存15h活力仍保持82%;"水池"内存在的1.0×10-3mol/L的Zn2+、Fe3+、Mg2+等离子对漆树酶有一定的抑制作用。漆树酶对邻氨基苯酚在反胶团和水溶液介质中,其主要氧化产物均为2-氨基-吩嗪-3-酮,但在反胶团中其反应产率比在水溶液中约高0.5倍。

关键词: 反胶团, 漆树酶, 氧化性能

Abstract: The oxidation of o-aminophenol catalyzed by Rhus Laccase(R. L.) solubilized in AOT/n-octane/H2O reverse micellar solution was studied. The optimal conditions observed for R. L. in reverse micelles are T=40℃, pH=7. 6 and R =[H2O]/[AOT]≈18. It was found that in reverse micelles at 30℃ for 15h in R=6. 2, the residual activity of R. L. re mains as high as 82%, and a little inhibition occurs when 1. 0×10-3mol/L Zn2+, Fe3+,Mg2+ exist in water pools. o-Aminophenol was oxidized by R. L. in reverse micelles and aqueous solution respectively,and it indicated that both of the main oxidation products are 2 amino-3-phenoxazinone. However, the yield of the oxidation product in the reverse micelles is 1.5 times as much as that in the aqueous solution.

Key words: Reverse micelles, Rhus laccase, Oxidation performance

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