高等学校化学学报

高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (2): 184.

• 论文 • 上一篇    下一篇

DTPA-Eu3+螯合物在核酸杂交分析中的应用

慈云祥, 陈勇, 李元宗, 常文保   

  1. 北京大学化学系, 北京 100871
  • 收稿日期:1994-04-07 修回日期:1994-09-10 出版日期:1995-02-24 发布日期:1995-02-24
  • 通讯作者: 慈云祥,男,67岁,教授,博士导师.
  • 作者简介:慈云祥,男,67岁,教授,博士导师.
  • 基金资助:

    国家自然科学基金

The Application of DTPA-Eu3+Complex to DNA Hybridization Assay

CI Yun-Xiang, CHEN Yong, LI Yuan-Zong, CNANG Wen-Bao   

  1. Deparment of Chemistry, Peking University, Beijing, 100871
  • Received:1994-04-07 Revised:1994-09-10 Online:1995-02-24 Published:1995-02-24

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摘要： 报道了靶DNA/生物素化λDNA探针/亲合素/Bio-BSA(TG)-DTPA-Eu3+体系在核酸杂交分析中的应用.亲合素做为连结杂交体与Bio-BSA-DTPA-Eu3+或Bio-TG-DTPA-Eu3+的桥,生物素和DTPA-Eu3+均标记在载体蛋白(BSA,TG)上.优化了分析条件,比较了己二胺、乙二胺转胺化探针及载体蛋白对杂交分析的影响.方法灵敏度高(1pg/well靶λDNA),重现性较好(RSD=5.7%,n=6).

关键词: 杂交分析, DTPA-Eu3+, 时间分辨荧光光度法

Abstract: Here we reports a new detection method for λDNA through the system of target DNA/Bio-DNA probe/Avidin/ Bio-BSA(TG)-DTPA-Eu3+.Avidin was used as a bridge to link hybrids and Bio-BSA-DTPA-Eu3+or Bio-TG-DTPA-Eu3+.The carrier proteins were labeled with both biotin and DTPA-Eu3+.The analytical conditions were optimized.The influences of transamination of DNA probe by ethylene diamine and 1,6-hexane diamine as well as carrier proteins on hybridization assay were compared.The method has a high sensitivity(1pg/well) with good reproducibility(RSD=5.7%,n=6).

Key words: Hybridization assay, DTPA-Eu3+, Time resolved fluorometry

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