高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (11): 1685.

• 论文 • 上一篇    下一篇

疏水作用色谱介质的合成及色谱特性研究

李华儒, 陈国亮   

  1. 西北大学化工系, 西安, 710069
  • 收稿日期:1994-11-19 修回日期:1995-03-08 出版日期:1995-11-24 发布日期:1995-11-24
  • 通讯作者: 李华儒,男,54岁,教授.
  • 作者简介:李华儒,男,54岁,教授.

Synthesis of HIC Packing and Study of Its Chromatographic Characteristics

LI Hua-Ru, CHEN Guo-Liang   

  1. Department of Chemical Engineering, Northwest University, Xi'an, 710069
  • Received:1994-11-19 Revised:1995-03-08 Online:1995-11-24 Published:1995-11-24

摘要: 利用国产大孔硅胶作基质合成了疏水填料。按照高效疏水作用色谱法,采用梯度洗脱方式分离了6种标准蛋白及唾液中α-淀粉酶和基因工程生产的γ-干扰素。柱子不可逆吸附小、被试验的α-淀粉酶和溶菌酶活性几乎定量被回收。应用合成的色谱填料研究了洗脱剂中盐浓度和温度对蛋白质保留行为的影响,论证了合成填料的色谱属性。

关键词: 高效疏水作用色谱, 填料合成, 色谱特性

Abstract: A HIC support has been synthesized by using the domestic silica with major pore Diameter. The mixture of six standard proteins,α-Amylase in saliva and rIFN-γ from E. Coli.were separated through gradient elution by high-performance hydrophobic interaction chromatograply respectively.The chromatographic column has less irreversible adsorption,α-Amylase and lysozyme used in this study are almost quantitatively recovered on thiscolumn. The effects of salt concentration in eluent and temperature on retention behaviours of proteins were investigated with packing prepared,and the chromatographic attribution of the support has been demonstrated.

Key words: High-performance hydrophobic interaction chromatography, Synthesis of packing, Chromatographic characteristics

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