计算机图象识别辅助菌种筛选及对甾体激素微生物酶反应的研究

高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (5): 750.

计算机图象识别辅助菌种筛选及对甾体激素微生物酶反应的研究

王积分¹, 阳葵², 刘宏¹, 安中¹, 段世铎²

1. 天津大学计算机系, 天津, 300072;
2. 天津大学化学系, 天津, 300072

收稿日期:1993-07-09 修回日期:1994-01-25 出版日期:1994-05-24 发布日期:1994-05-24
通讯作者: 段世铎
作者简介:王积分,女,55岁,教授.
基金资助:
国家自然科学基金

Studies on Seed Selection and Kinetic Model of Microorganism Enzymatic Steroid Transformation Using Computer Image Texture Analysis Technique

WANG Ji-Fen¹, YANG Kui², LIU Hong¹, AN Zhong¹, DUAN Shi-Duo²

1. Department of Computer, Tianjin University, Tianjin, 300072;
2. Department of Chemistry, Tianjin University, Tianjin, 300072

Received:1993-07-09 Revised:1994-01-25 Online:1994-05-24 Published:1994-05-24

摘要/Abstract

摘要： 将分形理论和计算机图象纹理分析技术移植到微生物发酵研究中,对菌落和菌丝体形态的定量描述进行了探讨。并利用菌种的分维特征作为筛选高产菌株的直观指标,有效地提高了菌种筛选工作的效率与准确度。探讨了沃氏底物霉菌转化的工艺过程,并以生物全息观念与分形理论为依托,在分形尺度上对生物体形态及其发育过程进行了描述,建立起菌体生长的形态动力学模型,并与生长(浓度)动力学模型相结合,设计出可判别理论最佳投料点的酶活描述模型。

关键词: 计算机图象分析, 分形模型, 微生物酶反应动力学, 菌种筛选, 菌体形态

Abstract: In this paper we employ Fractal Theory Organisms Full Information Law and Computer Texture Analysis Technique, and systematically investigate the characterization of morphologies of colonies and mycelium during the microorganism fermentation process. We demonstrate that using the fractal dimension characteristics to assist the mould seed selection can significantly improve the efficiency and accuracy. Moreover,we show that the morphology kinetic model and enzymatic activity model developed by us are capable of describing the microorganisms growing and enzymatic transformation process with a good degree of accuracy. When applied to predict the proper time of putting the substrates into the reaction system, the result is found to be in good agreement with the experimental data.

Key words: Computer image texture analysis, Fractal model, Organoorganism enzymatic reaction kinetics, Mould seed selection, MorphoIogies of colonies and mycelium

TrendMD:

王积分, 阳葵, 刘宏, 安中, 段世铎. 计算机图象识别辅助菌种筛选及对甾体激素微生物酶反应的研究. 高等学校化学学报, 1994, 15(5): 750.

WANG Ji-Fen, YANG Kui, LIU Hong, AN Zhong, DUAN Shi-Duo. Studies on Seed Selection and Kinetic Model of Microorganism Enzymatic Steroid Transformation Using Computer Image Texture Analysis Technique. Chem. J. Chinese Universities, 1994, 15(5): 750.