高等学校化学学报

高等学校化学学报 ›› 1993, Vol. 14 ›› Issue (12): 1661.

• 研究论文 • 上一篇    下一篇

高效毛细管电泳、激光干涉检测快速分离二糖研究

任吉存1, 何金兰2, 邓延倬2, 程介克1   

  1. 1. 武汉大学化学系, 武汉 430072;
    2. 武汉大学分析测试科学系, 武汉 430072
  • 收稿日期:1992-12-31 修回日期:1992-03-12 出版日期:1993-12-24 发布日期:1993-12-24
  • 通讯作者: 邓延悼.
  • 基金资助:

    国家自然科学基金

Rapid Separation of Disaccharides by Capillary Zone Electrophoresis with Interference Refractive Index Detection

REN Ji-Cun1, HE Jin-Lan2, DENG Yan-Zhuo2, CHENG Jie-Ke1   

  1. 1. Department of Chemistry, Wuhan University, Wuhan, 430072;
    2. Departemtn of Analysis and Testing, Wuhan University, Wuhan, 430072
  • Received:1992-12-31 Revised:1992-03-12 Online:1993-12-24 Published:1993-12-24

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被引次数

21

摘要： 首次报道一种高效毛细管电泳、激光干涉折射检测快速分离二糖的新方法.详细研究了四硼酸钠溶液中,pH值、四硼酸钠浓度及有机添加剂对二糖-硼酸络离子迁移行为的影响,发现在pH为9.7的0.1mol/L四硼酸钠溶液中,10min内4种二糖得到基线分离.采用自制激光干涉型折射指数检测器,不需对二糖衍生化处理,可直接进行在柱检测.

关键词: 高效毛细管电泳, 激光干涉, 二糖

Abstract: Rapid separation of disaccharides by capillary zone electrophoresis in uncoating fused-silica column is presented.Using a pH 9.7, 0.1 mol/Lsodium tetraborate as the runing solution, underivatized disaccharides are transformed into negatively charged borate complexes.Detection is performed with an on-column laser interference refractive index detector (made in home).Four disaccharides are separated by CZEwithin 10 min.The effects of pH, sodium tetr-borete concentration and organic additives on the separation of disaccharides are discussed.

Key words: CZE, Laser interference, Disaccharides

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