高等学校化学学报 ›› 1993, Vol. 14 ›› Issue (11): 1526.

• 研究快报 • 上一篇    下一篇

流动注射分析血清中葡萄糖的电化学检测池

许宏鼎, 邹明珠, 曹占双, 潘书鸿   

  1. 吉林大学化学系, 长春 130023
  • 收稿日期:1993-05-27 修回日期:1993-09-08 出版日期:1993-11-24 发布日期:1993-11-24
  • 通讯作者: 许宏娥, 男, 57岁, 教授.
  • 作者简介:许宏娥, 男, 57岁, 教授.
  • 基金资助:

    国家自然科学基金

An Electrochemical Detector Cell for Flow Injection Analysis of Glucose in Serum

XU Hong-Ding, ZOU Ming-Zhu, CAO Zhan-Shuang, PAN Shu-Hong   

  1. Department of Chemistry, Jilin University, Changchun, 130023
  • Received:1993-05-27 Revised:1993-09-08 Online:1993-11-24 Published:1993-11-24

关键词: 血清中的葡萄糖, 流动注射分析, 电化学检测池

Abstract: An electrochemical detector cell for FIAof glucose in serum has been developed.It was a three electrodes system involved a homemade copper-PTFEcomposite plated film electrode (working) and other two electrodes, silver (reference) and platinum(auxiliary) wires.The carrier liquid for FIAwas 0.5 mol/L NaOHand flowed through the cell at 2.1 mL/min.The working electrode potential was set at 0.6 V(vs.reference) by potentiostat.Sampling 30 ftLtest solution which was 1% serum sample diluted with water, a peak current was recorded by chronome-try.The peak current was linearly related with the concentration of glucose in the range of 5 × 10-3-2×10-1 mmol/L.The analytical results from our method were pretty correlated to those from glucose oxidase-4-aminoanti-pyrine-phenol method(Trinder method).The regression equation from the results of twenty samples was Y=-0.039 + 1.005X, r=0.9999.It was shown that our method was speedy, cheap and reliable.

Key words: Glucose in serum, Flow injection analysis, Electrochemical detector cell

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