高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (9): 1265.

• 论文 • 上一篇    下一篇

高分子添加剂对纤维素酶糖化力的影响

冯雁, 靳小红, 李增吉, 曹淑桂, 程玉华   

  1. 吉林大学酶工程实验室、化学系, 长春, 130023
  • 收稿日期:1990-09-24 出版日期:1991-09-24 发布日期:1991-09-24
  • 通讯作者: 冯雁

The Effect of Macromolecule Additives on the Saccharification of Cellulase

Feng Yan, Jin Xiao-hong, Li Zeng-ji, Cao Shu-gui, Cheng Yu-hua   

  1. The Laboratory of Enzyme Engineering, Department of Chemistry, Jilin University, Changchun, 130023
  • Received:1990-09-24 Online:1991-09-24 Published:1991-09-24

摘要: 研究了几种高分子材料对纤维素酶水解作用的影响,强聚阳离子(PⅠ、PⅡ)、中强聚阴离子(PⅢ)高分子材料能促进酶的糖化作用,而强聚阴离子(PⅣ)高分子材料则不能,添加PⅠ(1.3×10-1mol/L)、PⅡ(1.3×103mol/L)、PⅢ(1.3×102mol/L)分别使纤维素酶棉花糖化力提高190%、45%、75%,初步探讨了高分子材料促进纤维素酶糖化力的机理。

关键词: 纤维素酶, 高分子材料, 糖化力

Abstract: The effect of several synthesized macromolecules on the saccharification of cellulase was researched. Strong polypositive ion (PⅠ, P Ⅱ), moderately polynegative ion (PⅢ) macromolecules could enhance the saccharification. But strong polynegative ion (PⅣ) macromolecules could not. PⅠ (1. 3×10-1mol/L), PE (1. 3×10-3mol/L) and PI (1. 3×10-2mol/L) made the saccharification of cellulase increased by 190%, 45%, 75% respectively. The mechanism of enhancement of saccharification by macromolecules was researched from the view point--the effects of macro-molecules on the activity of each component of cellulase and the adsorption amount of enzyme on the substrate.

Key words: Cellulase, Macromolecules, Saccharification

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