高等学校化学学报

高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (8): 1052.

• 论文 • 上一篇    下一篇

氨基酸-铜配位化合物的紫外检测高效液相色谱研究

达世禄, 李小佳   

  1. 武汉大学化学系, 武汉, 430071
  • 收稿日期:1990-04-13 出版日期:1991-08-24 发布日期:1991-08-24
  • 通讯作者: 达世禄
  • 基金资助:

    国家自然科学基金

Studies on Complex of Amino Acid-Copper by High-Performance Liquid Chromatography with UV-Detection

Da Shi-Lu, Li Xiao-jia   

  1. Department of Chemistry, Wuhan University, Wuhan, 430072
  • Received:1990-04-13 Online:1991-08-24 Published:1991-08-24

PDF (PC)

被引次数

10

摘要： 研究了氨基酸-铜配位化合物的紫外检测反相高效液相色谱分离、测定方法,阐明了色谱分离、检测机理,探讨了流动相pH值、己基磺酸盐、铜离子、缓冲液浓度等对溶质保留和紫外检测灵敏度的影响,确定了最佳色谱分离、检测条件,并用于生物样品分析。

关键词: 氯基酸-铜配合物, 反相液相色谱, 紫外检测

Abstract: A new reversed-phase liquid chromatographic method with UV-detection for the analysis of amino acid-copper complex without any pretreatment or derivatizatiojn is studied. The mechanism of chromatographic separation and detection is described. The effect of factors such as the concentration of hexanesulfonate, copper ions and buffer as well as pHvalues is discussed. Guidelines are given for optimization of the separation. The analysis of amino acid samples are examined.

Key words: Complex of amino acid-copper, Reversed-phase liquid chromatography, UV-detection

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