高等学校化学学报

高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (4): 469.

• 论文 • 上一篇    下一篇

电化学发光法测定发光标记试剂ABEI

姚晴1, 安镜如2   

  1. 1. 福建医学院化学教研室, 350004;
    2. 福州大学化学系
  • 收稿日期:1989-12-14 出版日期:1991-04-24 发布日期:1991-04-24
  • 通讯作者: 姚晴
  • 基金资助:

    国家自然科学基金

The Determination of Luminescence-Labelled Agent ABEI by Electrogenerated Chemiluminescence

Yao Qing1, An Jing-ru2   

  1. 1. Teaching and Kesearch Group of Chemistry, Fujian Medical College, Fuzhou, 350004;
    2. Department of Chemistry, Fuzhou University
  • Received:1989-12-14 Online:1991-04-24 Published:1991-04-24

PDF (PC)

被引次数

4

摘要： ABEI-[N-(4-氨基丁基)-N-乙基]-氯基-2,3-二氢吩噻嗪-1,4-二酮是化学发光免疫分析法(简称LIA)中最常用的发光标记试剂,但有关电化学发光免疫分析目前尚未见报道。本文利用自制的电化学发光仪,对ABEI和ABEI标记兔抗HCG的电化学发光行为进行了研究。提出了利用电化学发光进行免疫分析的新途径。

关键词: 电化学发光免疫分析, ABEl, HCG

Abstract: The electrogenerated chemiluminescence of ABEI and ABEI-HCG in H3BO3-KCl-Na2CO3buffer solution at pH 11. 06 was studied. Co(Ⅱ)(200～600 μg/L) is capable of catalyzing the ABEIluminescence when a pulse potential is applied to the electrodes. Antibody of HCGis combined with ABEIto obtain ABEI-IgG. The detection limit of ABEIand ABEI-IgGare 2. 0×10-11mol/Land 2. 1×10-10mol/L, respectively. The concentration of ABEI, from 1.0×10-10to 5.0×10-7mol/ Lis in a linear relationship with the intensity of light.

Key words: Electrogenerated chemiluminescent immunoassay, Aminobutyl ethylisoluminol, HCG

TrendMD: