高等学校化学学报 ›› 1989, Vol. 10 ›› Issue (6): 609.

• 研究论文 • 上一篇    下一篇

高效液相色谱法测定蜂王浆中特有成分10-羟基-2-癸烯酸的研究

张燮, 傅春艳, 杨任飞   

  1. 吉林大学化学系
  • 收稿日期:1988-02-29 出版日期:1989-06-24 发布日期:1989-06-24
  • 通讯作者: 张燮

Determination of Characteristic Composition 10-Hydroxy-2-Decenoic Acid in Royal Jelly by High Performance Liquid Chromatography

Zhang Xie, Fu Chunyan, Yang Renfei   

  1. Department of Chemistry, Jilin University, Changchun
  • Received:1988-02-29 Online:1989-06-24 Published:1989-06-24

摘要: 本文报道反相高效液相色谱法测定蜂王浆和蜂王浆制剂中的10-羟基-2-癸烯酸(10-HDA)的方法,用二氯甲烷从蜂王浆或蜂王浆制剂中萃取10-HDA,而后用HPLC则定,实验采用YWG-C18柱,35%乙醇水溶液为流动相,己二酸为内标,紫外检测器波长为212nm,加入法测回收率在95%以上。用此法测定了鲜蜂王浆和蜂王浆制剂中的10-HDA的含量。该法简单、快速,优于气相色谱法和薄层色谱法。

关键词: 高效液相色谱法, 10-羟基-2-癸烯酸, 蜂王浆, 人参蜂王浆

Abstract: Amethod for the quantitative determination of 10-hydroxy-2-decenoic acid (10-HDA) in royal jelly(RJ) and RJpreparation was established by high performance liquid chromatography (HPLC).The lo-HDAextracted with dichlo-romethane from royal jelly or royal jelly preparation was determined by HPLC. The system was recommended on a YWG-C18 column (φ4.6×250 mm) using 35% ethanol in water (pH = 2.5) as the mobile phase; detection of ultraviolet absorption was performed at 212 nm, and at the range of 0.16 AUFS; internal standard, adipic acid.This method was successfully applied to the determination of 10-HDAcontents in raw royal jelly, ginseng royal jelly and ginseng pilose antler royal jelly. It is simpler, more rapid than and superior to gas chroma-tographic and thin-layer chromatographic methods.

Key words: HPLC, 10-Hydroxy-2-decenoic acid, Royal jelly, Ginseng royal jelly

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