高等学校化学学报

高等学校化学学报 ›› 1989, Vol. 10 ›› Issue (4): 401.

• 研究论文 • 上一篇    下一篇

尿毒症患者血清中中分子物质的提取及性质的初步表征

袁直, 张霁, 赵芬芝, 宓怀风, 何炳林   

  1. 南开大学高分子研究所、化学系
  • 收稿日期:1987-09-04 出版日期:1989-04-24 发布日期:1989-04-24
  • 通讯作者: 袁直
  • 基金资助:

    中国科学院科学基金

Isolation and Preliminary Characterization of Properties of Middle Molecules from Sera of Uremic Patients

Yuan Zhi, Zhang Ji, Zhao Fenzhi, Mi Huaifeng, He Binglin   

  1. Institute of Polymer Chemistry, Nankai University Department of Chemistry, Nankai University, Tianjin
  • Received:1987-09-04 Online:1989-04-24 Published:1989-04-24

PDF (PC)

被引次数

10

摘要： 从尿毒症患者血清及腹透液中得到的中分子量物质,具有相同的凝胶色谱行为及紫外、红外吸收行为,它们在206和235nm处,有特征紫外吸收峰;在波数为3246,1516,1122和613cm-1处,有特征红外吸收峰。中分子物经离子交换色谱进一步分离,发现起决定紫外及红外吸收行为的成分集中于第3子峰;其自由氨基酸和肽类物质含量均低于3%。

关键词: 尿毒症, 中分子量物质, 腹膜透析(CAPD), 凝胶渗透色谱

Abstract: Middle molecules in sera of uremic patients (UMM) were isolated on Sephadex G-15 gel-filtration Chromatography eluted with triethylamine caibonate buffer. Dry sample of middle molecules could be obtained with lyophilization. From the dialysis fluids of the uremic patients treated with continuous ambulatory peritoneal dialysis (CAPD) the same UMM fractions could be obtained. The behaviours of gel-filtration chromatography, UVand JRabsorptions of UMM from the sera and the dialysis fluids of uremic patients were identical. In 206nm and 235 nm, there were 2 UVabsorption peaks and in 3246, 1516, 1122 and 613 cm-1there were 4 characteristic IR-absorption peaks which are not found in normal sera. The Compositions responsible for the behaviours of UV and IR absorptions are found in the third sub-peak after the middle molecules were further separated by ion-exchange chromatography. The contents of peptides and free amino acid from the sample of UMM were quantitatively analyzed. They are between one to three percent.

Key words: Uremic, Middle molecules, Continuous ambulatory peritoneal dialysis, Gel-filtration chromatography

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