高等学校化学学报

高等学校化学学报 ›› 1988, Vol. 9 ›› Issue (5): 461.

• 论文 • 上一篇    下一篇

TTT活泼酰胺法在固相肽合成中的应用——促生长激素释放肽的合成及其生物活性

薛楚标1, 李崇熙1, 邢其毅1, 董明辉2   

  1. 1. 北京大学化学系;
    2. 中国科学院动物研究所
  • 收稿日期:1986-12-17 出版日期:1988-05-24 发布日期:1988-05-24
  • 通讯作者: 李崇熙

The Use of TTT Active Amide Method in Solid-phase Peptide Synthesis——The Synthesis of the Growth Hormone Releasing Peptide and Its Biological Activity

Xue Chubiao1, Li Chongxi1, Xing Qiyi1, Dong Minghui2   

  1. 1. Departmene of Chemistry, Peking University, Beijinn;
    2. Institute of Zoology, Academia Sinica, Beijing
  • Received:1986-12-17 Online:1988-05-24 Published:1988-05-24

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摘要： 通过合成促生长激素释放肽,对四氢噻唑-2-硫酮(TTT)活泼酰胺法在固相肽合成中的应用进行了研究。实验结果表明,TTT活泼酰胺同载体上的氨基组分的缩合反应速度很大,每步缩合反应的产率很高(>99.3%),最终产品的纯度也很令人满意。此外,还报道了合成肽的生物活性。

关键词: 四氢噻唑-2-硫酮, 活泼酰胺, 固相肽合成, 促生长激素释放肽

Abstract: The use of tetrahydrothiazole-2-thione (TTT) active amide method in solid-phase peptide synthesis has been studied through the synthesis of the growth hormone releasing peptide using chloromethylated copoly (styrene-2% divinylben-zene) resin as polymeric support. It was found that the coupling reaction was fast, the yield in each coupling step was nearly quantitative, and the purity of the final crude peptide was satisfactory. The biological activity of the synthetic peptide is also presented.

Key words: Tetrahydrothiazole-2-thione, Active amide, Solid phase peptide synthesis, Growth hormone releasing peptide

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