均相伏安免疫法检测白喉类毒素和白喉抗毒素

高等学校化学学报 ›› 1987, Vol. 8 ›› Issue (9): 793.

均相伏安免疫法检测白喉类毒素和白喉抗毒素

胡荫华, 铁锋, 李庆满

西北大学化学系

收稿日期:1986-05-06 出版日期:1987-09-24 发布日期:1987-09-24
基金资助:
中国科学院科学基金

Homogeneous Voltammetric Immunoassay of Diphtheria Toxoid and Diphtheria Antitoxin

Hu Yinhua, Tie Feng, Li Qingman

Department of Chemistry, Northwest University, Xi'an

Received:1986-05-06 Online:1987-09-24 Published:1987-09-24

摘要/Abstract

摘要： 白喉类毒素在pH9.4的0.1MNH₄Cl-NH₃·H₂O底液中改性,产生还原峰和氧化峰,峰电位分别为-1.52V和-1.46V(vs.SCE)。用单扫示波极谱法得到还原(或氧化)峰的峰电流与白喉类毒素浓度在2.5-180μg/ml范围内均呈良好线性关系。用微分脉冲极谱法可检测浓度低至0.3μg/ml的白喉类毒素。由特异性免疫结合反应,微分脉冲极谱法测得白喉类毒素的峰电流降低值与白喉抗毒素浓度在2.5×10^-6-5×10^-5IU/ml范围内呈正比关系,可测定白喉抗毒素浓度。方法简便、快速、灵敏度较高,可均相测定。用氧化峰电流测定百日咳菌苗-白喉-破伤风类毒素混合剂中白喉类毒素的含量,结果满意。

Abstract: Diphtheria toxoid is denatured in 0.1M NE₄Cl-NH₃·H₂O basal solution with pH9.4, yielding a cathodic and an anodic polarographic peaks at-1.50 and-1.46 V(vs.SCE) respectively.The cathodic and anodic peak heights vary linearly with diphtheria toxoid concentration over the range of 2.5 to 180 μg/ml by single sweep polarography; and even as low as 0.3μg/ml, diphtheria toxoid could be detected by differential pulse polarography. The decreased peak current value of diphtheria toxoid is proportional to diphtheria antitoxin present due to immuno-chemical binding reaction in the range of 2.5×10^-6to 5×10^-5IU/ml,which determines diphtheria antitoxin level homogencausly. The assays are sensitive specific, resproducible in operation The anodic peak has been applied to the determination of diphtheria toxoid in the triple vaccine.

TrendMD:

胡荫华, 铁锋, 李庆满. 均相伏安免疫法检测白喉类毒素和白喉抗毒素. 高等学校化学学报, 1987, 8(9): 793.

Hu Yinhua, Tie Feng, Li Qingman . Homogeneous Voltammetric Immunoassay of Diphtheria Toxoid and Diphtheria Antitoxin. Chem. J. Chinese Universities, 1987, 8(9): 793.