葡萄糖氧化酶的固定化方法与改进

高等学校化学学报 ›› 1987, Vol. 8 ›› Issue (6): 562.

葡萄糖氧化酶的固定化方法与改进

李丽霞, 鲁格, 陈长治, 俞耀庭

南开大学分子生物学研究所

收稿日期:1986-01-08 出版日期:1987-06-24 发布日期:1987-06-24

Comparison of Different Methods of Immobilized Glucose Oxidase

Li Lixia, Lu Ge, Chen Changzhi, Yu Yaoting

Institute of Moleclar Biology, Nankai University, Tianjin

Received:1986-01-08 Online:1987-06-24 Published:1987-06-24

摘要/Abstract

摘要： 本文在固定化葡葡糖氧化酶方面,对几种固定化载体和方法做了最佳条件摸索和比较。改进了戊二醛偶联法,尝试用二乙醇胺先保护戊二醛一端,另一端与载体偶联,然后脱去保护基再偶联酶,效果较好。用该法制得固定化酶在所研究的三种方法中效果最好。固定化酶活力回收率比用聚丙烯酰胺包埋法高出一倍多,比用重氮盐共价键合法高出10%左右。

Abstract: In this study, the bcsi condition of immobilized glucose oxidase using different supports and different methods was studied, and compared. An improved method of glucose oxidase immobilization method using glutaric dialdehyde was studied.One end of the glutaric dialdehyde molecule was first protected by diethanol-amine and the other end was linked to the carrier. Then the protected end was hydrolysed and glucose oxidase was linked to it. Clucose oxidase prepared according to the above method had the highest activity. The enzyme activity was 10% more than the didzo-reaction method and twice nigher than the polyacrylamide entrapment method.

TrendMD:

李丽霞, 鲁格, 陈长治, 俞耀庭. 葡萄糖氧化酶的固定化方法与改进. 高等学校化学学报, 1987, 8(6): 562.

Li Lixia, Lu Ge, Chen Changzhi, Yu Yaoting . Comparison of Different Methods of Immobilized Glucose Oxidase. Chem. J. Chinese Universities, 1987, 8(6): 562.