高等学校化学学报

高等学校化学学报 ›› 1986, Vol. 7 ›› Issue (8): 745.

• 研究论文 • 上一篇    下一篇

液膜固定化细胞甾体转化的研究

姜坪, 李乃宏, 何炳林   

  1. 南开大学高分子化学研究所
  • 收稿日期:1985-04-25 出版日期:1986-08-24 发布日期:1986-08-24

Study on Steroid Transformation Catalyzed by Liquid Surfactant Membrane Encapsulated Cells

Jang Ping, Li Naihong, He Dinglin   

  1. Polymer Chemistry institute of Nankai University, Tianjin
  • Received:1985-04-25 Online:1986-08-24 Published:1986-08-24

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摘要： 本文研究了液膜固定化简单节杆菌进行较大有机分子甾体类的生物转化,结果表明液膜固定化细胞可成功进行氢化可的松→去氢氢化可的松的△′-脱氢反应。研究了液膜固定化细胞的制备、性质和影响甾体转化的各种因素。在某些液膜体系中其转化反应初期的比活力与自由细胞相同。细胞在液膜内可存活较长时间,提高了贮存稳定性。液膜固定化细胞体系基本不受染茵的影响,而自由细胞转化作用明显受抑制。

Abstract: In this paper it deseribes the preparation and properties of the encapsulated arthrobacter simplex cells.by liquid surfactant membrane which catalyzes the transformation of biger steroid molecule,i.e.,corlisol into prednisolone.The experimental results indicated that the catalytic transformation take place very well and in some system the encapsulated cells keep the initial rate of catalytic transformation as good as that of the free cells.By encapsulation it raises the stability of stroragc.We also investigated various factors which affected the biocatalytic transformation from which we knew that the encapsulated cells system was not influenced by the contaminaljon of Bacillous cells which inhibited the free cells system seriouslv.

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