用DNA-球形碳化聚合物吸附剂由血液灌流除去哺乳动物血中DNA抗体及其免疫复合物

高等学校化学学报 ›› 1985, Vol. 6 ›› Issue (9): 843.

用DNA-球形碳化聚合物吸附剂由血液灌流除去哺乳动物血中DNA抗体及其免疫复合物

杨彦¹, 李乃宏¹, 何炳林¹, 权武烈², 崔润海², 邵新华², 叶福华², 钱绍诚², 宋继昌²

1. 南开大学化学系, 南开大学分子生物学研究所;
2. 天津河东医院肝胆研究室

收稿日期:1984-06-16 出版日期:1985-09-24 发布日期:1985-09-24

Removal of DNA Antibodies and DNA Immune Complexes from Mammal Blood with Spherical Carbonaceous Polymeric Adsorbent by Hemoperfusion

Yang Yan¹, Li Naihong¹, He Binglin¹, Quan Wulie², Cui Runhai², Shao Xinhua², Ye Fuhua², Qian Shaocheng², Song Jichang²

1. Department of Chemistry, Nankai University, Institute of Molecular Biochemistry, Nankai University, Tianjin;
2. Liter and Biliary Disease Research Unit. Hedong Hospital, Tianjin

Received:1984-06-16 Online:1985-09-24 Published:1985-09-24

摘要/Abstract

摘要： DNA与兰四唑阳离子络合后与火棉胶混合最后吸附在大分子球形碳化聚合物吸附剂上,DNA固定的百分率是98—98.5％,它被应用到动物血液灌流能特异除去注入的DNA抗体及免疫复合物,功效在75％以上,而引起的非特异吸附仅低于10％,它有好的稳定性和血相容性,因此,它可能应用到临床治疗SLE上。

Abstract: DNAis complexed with tetrazolium cations (Blue tetrazolum, BT) and then mixed with collodion and finally adsorbed on the macroporous spherical carbonaceous polymeric adsorbents. The percentage of DNAthus immobilized is 98-98.5%. It was applied to animal hemoperfusion for the removal of the DNAantibodies and its immune complexes which were passively infused. After 10-20 minutes adsorption, a sharp reduction of DNAbinding was observed and the futuin (AFP) binding percentage only dropped slightly. The efficiency of the specific removal of DNAimmune complex was over 75% and only less than 10% unspecific adsorption occurred. Tt has a good stability and blood compatibility. Therefore, it might be applicable 1o clinical SLEtherapy.

TrendMD:

杨彦, 李乃宏, 何炳林, 权武烈, 崔润海, 邵新华, 叶福华, 钱绍诚, 宋继昌. 用DNA-球形碳化聚合物吸附剂由血液灌流除去哺乳动物血中DNA抗体及其免疫复合物. 高等学校化学学报, 1985, 6(9): 843.

Yang Yan, Li Naihong, He Binglin, Quan Wulie, Cui Runhai, Shao Xinhua, Ye Fuhua, Qian Shaocheng, Song Jichang. Removal of DNA Antibodies and DNA Immune Complexes from Mammal Blood with Spherical Carbonaceous Polymeric Adsorbent by Hemoperfusion. Chem. J. Chinese Universities, 1985, 6(9): 843.