高等学校化学学报 ›› 1985, Vol. 6 ›› Issue (6): 557.

• 研究论文 • 上一篇    下一篇

空间排阻色谱(SEC)用多孔硅胶载体的研究

汪勤慰, 段晓青, 敬志成, 哈惠荣   

  1. 北京大学化学系
  • 收稿日期:1984-02-24 出版日期:1985-06-24 发布日期:1985-06-24

The Study of Porous Silica Gel for Aqueous Size Exclusion Ghromatographic Use

Wang Qinwei, Duan Xiaoqing, Jing Zhicheng, Ha Huirong   

  1. Department of Chemistry, Peking University, Beijing
  • Received:1984-02-24 Online:1985-06-24 Published:1985-06-24

摘要: 我们的工作是制备多孔硅球表面键合带有羟基基团的亲水性SEC刚性载体.研究结果表明,此种SEC载体能适用于聚乙烯醇、明胶、蛋白质、右旋糖酐等多种水溶液体系的SEC分析.

Abstract: The preparation of a silica-based packing for the aqueous size Exclusion Chro-matography (SEC) is described. The process involved the hydrogen-bonding of a coupling reagent with the silica gel surface followed by ring-openning reactions of the epoxy groups on the coupling reagent molecules by polyethylene glycols. The results show that this modified packing can be used for the SECanalysis of polyvinyl acetate, gelatins, proteins and dextran systems.

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