黄素单核苷酸的脉冲极谱法研究及其在汞电极上反应速度常数的测定

高等学校化学学报 ›› 1985, Vol. 6 ›› Issue (11): 963.

黄素单核苷酸的脉冲极谱法研究及其在汞电极上反应速度常数的测定

宋鸿镃, 汪乃兴, 章作武

复旦大学化学系

收稿日期:1984-05-29 出版日期:1985-11-24 发布日期:1985-11-24

Studies on the Pulse Polarography of Flavin Mononucleotide and It's Measurement of Rate Constant at the Mercury Electrode

Song Hongzi, Wang Naixing, Zhang Zuowu

Department of Chemistry, Fudan University, Shanghai

Received:1984-05-29 Online:1985-11-24 Published:1985-11-24

摘要/Abstract

摘要： 本文研究了黄素单核苷酸的脉冲极谱行为,在选定的最佳条件下作了定量测定,检出限可达6.8×10^-7M,方法简便快速,本文还藉循环伏安法研究了黄素单核苷酸的电极反应动力学性质,测定了其在汞电极上的反应速度常数。

Abstract: The differential pulse polarographic behaviour of flavin mononucleotide (FMN) and determinative conditions have been investigated. The optimum conditions for the determination of FMNare as fllows: the clectrolyte was 0.01Msodium citrate (pH5), initial potential -0.100V (vs.Ag-AgCl), final potential - 0.600V(vs·Ag -AgCl), voltage sweep rate 100mV/sec. Afair reproducibility was obtained in the above progedure and a good proportionality between the values of the peak current and FMNconcentration (1.8×10^-6-4.7×10^-5M) has been observed. The decection limit is 6.8×10^-7M. This method provides a rapid and simple way to determination of FMN.This paper also reports that the application of cyclic voltammelry to study electrode process kinetic behavior has been established. Under the experimental conditions the rate constants for electron transfer arc determined. The values of k arc in good agreement with literature's results.

TrendMD:

宋鸿镃, 汪乃兴, 章作武. 黄素单核苷酸的脉冲极谱法研究及其在汞电极上反应速度常数的测定. 高等学校化学学报, 1985, 6(11): 963.

Song Hongzi, Wang Naixing, Zhang Zuowu . Studies on the Pulse Polarography of Flavin Mononucleotide and It's Measurement of Rate Constant at the Mercury Electrode. Chem. J. Chinese Universities, 1985, 6(11): 963.