Abstract: The study of the fatty acids contents in Camellia weiningensis Li sp. nov (Theaceae) seed oil was carried out by using two analytical methods.The unsaponifiables of this oil were separated by silica gel column chromatography into four fractions. The present paper deals with the composition of triterpene alcohols, 4-methylsterols and sterols. All these fractions were analysed by TLCand GLCtheir major mponents were identified by melting point determination, optical rotation, GLC, TLC, IRand MSa-Spinasterol, △⁷-stigmastenol, β-amyrin, germanicol, ψ-taraxasterol, tirucalladienol,butyrospermol and dammaradienol were isolated and identified. ⁷-Avenasterol, a-amyrin, citrostadienol and cycloeucalenol were identified. Trace quantities of △⁵-sterols were also separated by designed TLCon silica gel and cholesterol, campesterol, stigmasterol and β-sitosterol were identified.No evidence of nonedibles was found in this oil.