高等学校化学学报 ›› 1981, Vol. 2 ›› Issue (4): 423.

• 论文 • 上一篇    下一篇

顺-[Pt(NH3)2Cl2]与核苷的作用

唐雯霞, 张才芬, 戴安邦   

  1. 南京大学络合物化学研究所
  • 收稿日期:1980-11-28 出版日期:1981-12-24 发布日期:1981-12-24

THE REACTION OF cis-[Pt(NH3)2Cl2] ON NUCLEOSIDES

Tang Wenxia, Zhang Caifen, Tai An-pang   

  1. Coordination Chemistry Institute, Nanjing University, Nanjing
  • Received:1980-11-28 Online:1981-12-24 Published:1981-12-24

摘要: 用分光光度法研究了37℃、pH=5.5、0.1M NaClO4介质中cis[Pt(NH3)2Cl2]和DNA组成物——鸟嘌呤核苷、腺嘌呤核苷、胞嘧啶核苷及胸腺嘧啶核苷的作用。发现顺-[Pt(NH3)2]与前三种核苷能生成组成为1:1、1:2二种络合物,与胸腺嘧啶核苷不作用。所测得一级和二级表观生成常数,以及作用初速分别有如下大小次序:Guo>Ado>Cyt》Thy;Guo>Ado>Cyt》Thy.在所得结果基础上讨论了顺-[Pt(NH3)2Cl2]和癌细胞中DNA作用的可能方式。

Abstract: The interaction of cis-[Pt(NH3)2Cl2] with guanosine, adenosine, cyti-dine and thymidine, as constituents of DNA, was studied spectrophotome-trically at pH5.5,37°C in the presence of 0.1M NaClO4. It was found that the first three nucleosides formed 1:1 and 1:2 complexes with cis-CptⅡ (NH3)2], whose apparent stability constants and initial rate of formation are given as follows:Thymidine showed no interaction with cis-[PtⅡ (NH3)2].On the basis of the above findings, a possible mode of reaction of cis-[Pt(NH3)2Cl2] with DNA in the cancer cells is discussed. Thermodynami-cally and kinetically, it is considered most probable that cross links were formed between cis-[PtⅡ (NH3)2] and nucleosides in DNA, e. g.,guanosine,and it seems unlikely that a chelate was formed withany one nucleoside, e. g.,

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