高等学校化学学报 ›› 2023, Vol. 44 ›› Issue (3): 20220319.doi: 10.7503/cjcu20220319

• 综合评述 • 上一篇    下一篇

N4-乙酰胞苷RNA检测技术的研究进展

贺胤铭1,4, 孔素东2,3, 林建国2,3, 谢敏浩2,3, 程靓1,4()   

  1. 1.中国科学院化学研究所, 北京分子科学国家研究中心, 中国科学院分子科学科教融合卓越创新中心,分子识别与功能重点实验室, 北京 100190
    2.江南大学化学与材料工程学院, 无锡 214122
    3.国家卫生健康 委员会核医学重点实验室, 江苏省原子医学研究所, 无锡 214063
    4.中国科学院大学, 北京 100049
  • 收稿日期:2022-05-10 出版日期:2023-03-10 发布日期:2023-03-14
  • 通讯作者: 程靓 E-mail:chengl@iccas.ac.cn
  • 作者简介:第一联系人:共同第一作者.
  • 基金资助:
    国家自然科学基金(22022704)

Research Advances of Detection Approaches Towards N4-Acetylcytidine(ac4C) RNA

HE Yinming1,4, KONG Sudong2,3, LIN Jianguo2,3, XIE Minhao2,3, CHENG Liang1,4()   

  1. 1.Beijing National Laboratory for Molecular Sciences(BNLMS),CAS Key Laboratory of Molecular Recognition and Function,CAS Research/Education Center for Excellence in Molecular Sciences,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China
    2.School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,China
    3.NHC Key Laboratory of Nuclear Medicine,Jiangsu Institute of Nuclear Medicine,Wuxi 214063,China
    4.University of Chinese Academy of Sciences,Beijing 100049,China
  • Received:2022-05-10 Online:2023-03-10 Published:2023-03-14
  • Contact: CHENG Liang E-mail:chengl@iccas.ac.cn
  • Supported by:
    the National Natural Science Foundation of China(22022704)

摘要:

细胞中的mRNA和非编码RNA包含着大量的表观化学修饰. 在这些修饰中, N4-乙酰胞苷(ac4C)是较为独特的一种, 在真核生物和原核生物的tRNA, rRNA和mRNA中均有发现. 研究表明, ac4C RNA可能具有多种生物学功能, 包括调节蛋白的翻译过程、 影响RNA的稳定性及改变RNA-蛋白相互作用等. 但当前对其修饰路径的研究还不成熟, 催化ac4C RNA形成的乙酰转移酶目前仅有NAT10被鉴定出来. 不仅如此, 目前对ac4C RNA进行检测和测序的技术手段还相当局限. 本文综合评述了ac4C RNA的分布以及在基因表达调控中的作用和机制, 重点介绍了ac4C RNA的检测技术, 并对ac4C RNA当前研究中的不足和机遇进行了总结和展望.

关键词: N4-乙酰胞苷, RNA修饰, 检测方法

Abstract:

A large number of epigenetic chemical modifications have been detected in cellular mRNA and non-coding RNAs. Among these modifications, N4-acetylcytidine(ac4C) is a relatively unique one, which has widely been discovered in tRNA, rRNA and tRNAs in eukaryotes and prokaryotes. Current research have shown that ac4C RNA may possess a variety of biological functions, including regulating the translation process of proteins, affecting the stability of RNA, and modulating RNA-protein interactions. However, only one ac4C acetyltransferase, NAT10, has been identified so far. Besides, current technical methods for the detection and sequencing of ac4C RNA are quite limited. This review comprehensively summarized current advances in the exploration of distribution of ac4C RNA, its roles in gene expression regulation and its detection methods. Finally the opportunities and shortcomings of the related research on ac4C were summarized and prospected.

Key words: N4-Acetylcytidine, RNA modification, Detection method

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